Recombinant Anti-LDL Receptor antibody [EP1553Y] (ab52818)
![Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-383729-ab52818-anti-ldl-receptor-western-blot-pc3-lncap-huh7-hepg2-hela-a431-hek293.jpg "Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1553Y] to LDL Receptor
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-LDL Receptor antibody [EP1553Y]
See all LDL Receptor primary antibodies -
Description
Rabbit monoclonal [EP1553Y] to LDL Receptor -
Host species
Rabbit -
Specificity
Some optimisation may be required for detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for suitable positive controls.
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Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human LDL Receptor aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P01130 -
Positive control
- WB: HeLa and RAW264.7 cell lysate. HepG2 whole cell lysate. Mouse liver, lung and colon lysate. Human liver and plasma lysate. IHC-P: Human hepatocellular carcinoma and liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1553Y -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB | (7) |
1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
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| Notes |
|---|
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa). |
Application notes
Is unsuitable for Flow Cyt or ICC/IF.
Target
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Function
Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. -
Involvement in disease
Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). -
Sequence similarities
Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats. -
Post-translational
modificationsN- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation. -
Cellular localization
Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments. - Information by UniProt
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Database links
- Entrez Gene: 3949 Human
- Entrez Gene: 16835 Mouse
- Omim: 606945 Human
- SwissProt: P01130 Human
- SwissProt: P35951 Mouse
- Unigene: 213289 Human
- Unigene: 728190 Human
- Unigene: 3213 Mouse
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Alternative names
- FH antibody
- FHC antibody
- LDL R antibody
see all
Images
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Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution
Lane 1 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
Lane 3 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 5 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 7 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-255277-anti-ldl-receptor-antibody-ep1553y-western-blot.jpg)
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa treated with GW3965 for 8 hours at the final concentration of 5uM whole cell lysates
Lane 3 : HeLa treated with GW3965 for 24 hours at the final concentration of 5uM whole cell lysates
Lane 4 : Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysates
Lane 5 : Raw264.7 treated with GW3965 for 8 hours at the final concentration of 5uM whole cell lysates
Lane 6 : Raw264.7 treated with GW3965 for 24 hours at the final concentration of 5uM whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))
Predicted band size: 95 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking buffer: 5% NFDM/TBST
Diltuion buffer: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-261846-anti-ldl-receptor-antibody-ep1553y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)Immunohistochemical analysis of paraffin-embedded human liver sections labeling LDL Receptor with purified ab52818 at dilution of 1:500. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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![Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-308389-anti-ldl-receptor-antibody-ep1553y-western-blot.jpg)
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 0.4 µg/ml + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 95 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with the literature (PMID: 15199428, PMID: 8349823, PMID: 10906332, PMID: 24918045). -
![Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-255276-anti-ldl-receptor-antibody-ep1553y-western-blot.jpg)
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse lung lysate
Lane 3 : Mouse colon lysate
Lane 4 : Human liver lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (HRP goat anti-rabbit IgG (H+L))
Predicted band size: 95 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking buffer: 5% NFDM/TBST
Diltuion buffer: 5% NFDM/TBST
-
![Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-261833-anti-ldl-receptor-antibody-ep1553y-western-blot.jpg)
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/5000 dilution + Mouse liver at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Predicted band size: 95 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer 5% NFDM/TBST -
![Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-65258-anti-ldl-receptor-antibody-ep1553y-western-blot.jpg)
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/500 dilution + Human plasma total protein lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 27 kDa, 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
LDL Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-255275-anti-ldl-receptor-antibody-ep1553y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling LDL Receptor with ab52818 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counterstained with hematoxylin.
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![Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-19-benefits-of-recombinant-antibodies.png)
Anti-LDL Receptor antibody [EP1553Y] (ab52818)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (100)
ab52818 has been referenced in 100 publications.
- Wang S et al. AXL is a candidate receptor for SARS-CoV-2 that promotes infection of pulmonary and bronchial epithelial cells. Cell Res 31:126-140 (2021). PubMed: 33420426
- Li Z et al. Exosome-based Ldlr gene therapy for familial hypercholesterolemia in a mouse model. Theranostics 11:2953-2965 (2021). PubMed: 33456582
- Emmer BT et al. Genome-scale CRISPR screening for modifiers of cellular LDL uptake. PLoS Genet 17:e1009285 (2021). PubMed: 33513160
- Xu M et al. Development of a novel, fully human, anti-PCSK9 antibody with potent hypolipidemic activity by utilizing phage display-based strategy. EBioMedicine 65:103250 (2021). PubMed: 33647772
- Lizardo K et al. Fat tissue regulates the pathogenesis and severity of cardiomyopathy in murine chagas disease. PLoS Negl Trop Dis 15:e0008964 (2021). PubMed: 33826636