Anti-LDL Receptor antibody [EP1553Y] (ab52818)
- Datasheet
- Specific References (42)
- Protocols
Overview
-
Product nameAnti-LDL Receptor antibody [EP1553Y]
See all LDL Receptor primary antibodies -
DescriptionRabbit monoclonal [EP1553Y] to LDL Receptor
-
Host speciesRabbit
-
Tested applicationsSuitable for: ICC/IF, IHC-P, WB, Flow Cytmore details
-
Species reactivityReacts with: Mouse, Human
-
Immunogen
Synthetic peptide within Human LDL Receptor (C terminal). The exact sequence is proprietary.
-
Positive control
- HepG2 cells and cell lysate
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.1% BSA -
Concentration information loading... -
PurityProtein A purified
-
ClonalityMonoclonal
-
Clone numberEP1553Y
-
IsotypeIgG
-
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
-
Alternative Versions
- Anti-LDL Receptor antibody [EP1553Y] (HRP) (ab195516)
- Anti-LDL Receptor antibody [EP1553Y] (Alexa Fluor® 647) (ab196160)
- Anti-LDL Receptor antibody [EP1553Y] (Alexa Fluor® 488) (ab196377)
- Anti-LDL Receptor antibody [EP1553Y] - Low endotoxin, Azide free (ab215980)
- Anti-LDL Receptor antibody [EP1553Y] (Allophycocyanin) (ab224989)
-
Compatible Secondaries
-
Conjugation kits
-
Immunohistochemistry kits
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab52818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| IHC-P | 1/500. | |
| WB | 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa). | |
| Flow Cyt | 1/70 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
FunctionBinds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
-
Involvement in diseaseDefects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).
-
Sequence similaritiesBelongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats. -
Post-translational
modificationsN- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation. -
Cellular localizationCell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments.
- Information by UniProt
-
Database links
- Entrez Gene: 3949 Human
- Entrez Gene: 16835 Mouse
- Omim: 606945 Human
- SwissProt: P01130 Human
- SwissProt: P35951 Mouse
- Unigene: 213289 Human
- Unigene: 728190 Human
- Unigene: 3213 Mouse
-
Alternative names
- FH antibody
- FHC antibody
- LDL R antibody
see all
Images
-
![Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-255277-ab52818WB2.jpg)
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa treated with GW3965 for 8 hours at the final concentration of 5uM whole cell lysates
Lane 3 : HeLa treated with GW3965 for 24 hours at the final concentration of 5uM whole cell lysates
Lane 4 : Raw264.7 whole cell lysates
Lane 5 : Raw264.7 treated with GW3965 for 8 hours at the final concentration of 5uM whole cell lysates
Lane 6 : Raw264.7 treated with GW3965 for 24 hours at the final concentration of 5uM whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))
Predicted band size: 95 kDa
Observed band size: 140 kDa (why is the actual band size different from the predicted?)
Exposure time: 1 minuteBlocking buffer: 5% NFDM/TBST
Diltuion buffer: 5% NFDM/TBST
-
![Immunocytochemistry/ Immunofluorescence - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-15-ab52818ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LDL Receptor antibody [EP1553Y] (ab52818)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LDL Receptor with purified ab52818 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor®594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei were counterstained with DAPI (blue).
For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (ab150120) were used. For negative control 2, mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) were used.
-
![Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-308389-ab52818WB.jpg)
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 0.4 µg/ml + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 95 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with the literature (PMID: 15199428, PMID: 8349823, PMID: 10906332, PMID: 24918045). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-14-ab52818IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)Immunohistochemical analysis of paraffin-embedded human liver sections labellingLDL Receptor with purified ab52818 at dilution of 1:500. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
-
![Flow Cytometry - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-16-ab52818FC1.jpg)
Flow Cytometry - Anti-LDL Receptor antibody [EP1553Y] (ab52818)Overlay histogram showing 4% paraformaldehyde fixed HeLa cells labelling LDL Receptor (red) with purified ab52818 at dilution of 1/70. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG at dilution of 1/2000. A non-specific IgG antibody (rabbit monoclonal) was used as isotype control (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
-
![Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-255276-ab52818WB1.jpg)
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution
Lane 1 : mouse liver lysate
Lane 2 : mouse lung lysate
Lane 3 : mouse colon lysate
Lane 4 : human liver lysate
Lane 5 : HepG2 cell lysate
Lane 6 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (HRP goat anti-rabbit IgG (H+L))
Predicted band size: 95 kDa
Observed band size: 140 kDa (why is the actual band size different from the predicted?)
Exposure time: 3 minutesBlocking buffer: 5% NFDM/TBST
Diltuion buffer: 5% NFDM/TBST
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-255275-52818IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling LDL Receptor with ab52818 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
-
![Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-13-ab52818WB2.jpg)
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/5000 dilution + Mouse liver at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Predicted band size: 95 kDa
Observed band size: 140 kDa (why is the actual band size different from the predicted?)
Blocking/Diluting buffer 5% NFDM/TBST -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/ab52818-255274-52818IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling LDL Receptor with ab52818 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
-
![Immunocytochemistry/ Immunofluorescence - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/LDL-Receptor-Primary-antibodies-ab52818-4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LDL Receptor antibody [EP1553Y] (ab52818)ICC/IF image of ab52818 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52818, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Flow Cytometry - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/LDL-Receptor-Primary-antibodies-ab52818-3.jpg)
Flow Cytometry - Anti-LDL Receptor antibody [EP1553Y] (ab52818)Overlay histogram showing U937 cells stained with ab52818 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52818, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)](https://www.abcam.com/ps/products/52/ab52818/Images/LDL-Receptor-Primary-antibodies-ab52818-2.jpg)
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/500 dilution + Human Plasma Total Protein Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 100 kDa (why is the actual band size different from the predicted?)
Additional bands at: 27 kDa, 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
LDL Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
References
This product has been referenced in:
- Zhu L et al. Hepatocyte estrogen receptor alpha mediates estrogen action to promote reverse cholesterol transport during Western-type diet feeding. Mol Metab 8:106-116 (2018). WB . Read more (PubMed: 29331506) »
- Xie Z et al. Adipose-Derived Exosomes Exert Proatherogenic Effects by Regulating Macrophage Foam Cell Formation and Polarization. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29502100) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"