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    ldl-receptor-antibody-ep1553y-ab52818.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism
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RecombinantRabMAb

Recombinant Anti-LDL Receptor antibody [EP1553Y] (ab52818)

  • Datasheet
  • SDS
Reviews (16)Q&A (8)References (100)

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Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Anti-LDL Receptor antibody [EP1553Y] (ab52818)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1553Y] to LDL Receptor
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

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Overview

  • Product name

    Anti-LDL Receptor antibody [EP1553Y]
    See all LDL Receptor primary antibodies
  • Description

    Rabbit monoclonal [EP1553Y] to LDL Receptor
  • Host species

    Rabbit
  • Specificity

    Some optimisation may be required for detection of the target protein due to low levels of endogenous expression in some samples.  Please see images below for suitable positive controls.

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human LDL Receptor aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P01130

  • Positive control

    • WB: HeLa and RAW264.7 cell lysate. HepG2 whole cell lysate. Mouse liver, lung and colon lysate. Human liver and plasma lysate. IHC-P: Human hepatocellular carcinoma and liver tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1553Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Second Messenger
    • Lipid Messengers
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • HRP Anti-LDL Receptor antibody [EP1553Y] (ab195516)
    • Anti-LDL Receptor antibody [EP1553Y] - Low endotoxin, Azide free (ab215980)
    • Anti-LDL Receptor antibody [EP1553Y] - BSA and Azide free (ab271846)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human LDL Receptor protein (Active) (ab220570)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocol.

WB (7)
1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
Notes
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocol.

WB
1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
  • Involvement in disease

    Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).
  • Sequence similarities

    Belongs to the LDLR family.
    Contains 3 EGF-like domains.
    Contains 7 LDL-receptor class A domains.
    Contains 6 LDL-receptor class B repeats.
  • Post-translational
    modifications

    N- and O-glycosylated.
    Ubiquitinated by MYLIP leading to degradation.
  • Cellular localization

    Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments.
  • Target information above from: UniProt accession P01130 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3949 Human
    • Entrez Gene: 16835 Mouse
    • Omim: 606945 Human
    • SwissProt: P01130 Human
    • SwissProt: P35951 Mouse
    • Unigene: 213289 Human
    • Unigene: 728190 Human
    • Unigene: 3213 Mouse
    • Alternative names

      • FH antibody
      • FHC antibody
      • LDL R antibody
      • LDL receptor antibody
      • LDLCQ2 antibody
      • Ldlr antibody
      • LDLR_HUMAN antibody
      • Low Density Lipoprotein Receptor antibody
      • Low density lipoprotein receptor class A domain containing protein 3 antibody
      • Low density lipoprotein receptor familial hypercholesterolemia antibody
      • Low-density lipoprotein receptor antibody
      see all

    Images

    • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution

      Lane 1 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
      Lane 3 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
      Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
      Lane 5 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 6 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
      Lane 7 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

      Predicted band size: 95 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 2 : HeLa treated with GW3965 for 8 hours at the final concentration of 5uM whole cell lysates
      Lane 3 : HeLa treated with GW3965 for 24 hours at the final concentration of 5uM whole cell lysates
      Lane 4 : Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysates
      Lane 5 : Raw264.7 treated with GW3965 for 8 hours at the final concentration of 5uM whole cell lysates
      Lane 6 : Raw264.7 treated with GW3965 for 24 hours at the final concentration of 5uM whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size: 95 kDa
      Observed band size: 140 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking buffer: 5% NFDM/TBST

      Diltuion buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)

      Immunohistochemical analysis of paraffin-embedded human liver sections labeling LDL Receptor with purified ab52818 at dilution of 1:500. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 0.4 µg/ml + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 95 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with the literature (PMID: 15199428, PMID: 8349823, PMID: 10906332, PMID: 24918045).

    • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution

      Lane 1 : Mouse liver lysate
      Lane 2 : Mouse lung lysate
      Lane 3 : Mouse colon lysate
      Lane 4 : Human liver lysate
      Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
      Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size: 95 kDa
      Observed band size: 140 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking buffer: 5% NFDM/TBST

      Diltuion buffer: 5% NFDM/TBST

    • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/5000 dilution + Mouse liver at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

      Predicted band size: 95 kDa
      Observed band size: 140 kDa why is the actual band size different from the predicted?



      Blocking/Diluting buffer 5% NFDM/TBST
    • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/500 dilution + Human plasma total protein lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 95 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?
      Additional bands at: 27 kDa, 48 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 4 minutes


      LDL Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)

      Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling LDL Receptor with ab52818 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counterstained with hematoxylin.

    • Anti-LDL Receptor antibody [EP1553Y] (ab52818)
      Anti-LDL Receptor antibody [EP1553Y] (ab52818)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (100)

    Publishing research using ab52818? Please let us know so that we can cite the reference in this datasheet.

    ab52818 has been referenced in 100 publications.

    • Wang S  et al. AXL is a candidate receptor for SARS-CoV-2 that promotes infection of pulmonary and bronchial epithelial cells. Cell Res 31:126-140 (2021). PubMed: 33420426
    • Li Z  et al. Exosome-based Ldlr gene therapy for familial hypercholesterolemia in a mouse model. Theranostics 11:2953-2965 (2021). PubMed: 33456582
    • Emmer BT  et al. Genome-scale CRISPR screening for modifiers of cellular LDL uptake. PLoS Genet 17:e1009285 (2021). PubMed: 33513160
    • Xu M  et al. Development of a novel, fully human, anti-PCSK9 antibody with potent hypolipidemic activity by utilizing phage display-based strategy. EBioMedicine 65:103250 (2021). PubMed: 33647772
    • Lizardo K  et al. Fat tissue regulates the pathogenesis and severity of cardiomyopathy in murine chagas disease. PLoS Negl Trop Dis 15:e0008964 (2021). PubMed: 33826636
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 24 Abreviews or Q&A

    Western blot abreview for Anti-LDL Receptor antibody [EP1553Y]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Dopaminergic Neurons & HEK-293T)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Dopaminergic Neurons & HEK-293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 16 2020

    Western blot abreview for Anti-LDL Receptor antibody [EP1553Y]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (kidney)
    Gel Running Conditions
    Reduced Denaturing (4-12% bis-tris gel)
    Loading amount
    30 µg
    Specification
    kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 03 2020

    Western blot abreview for Anti-LDL Receptor antibody [EP1553Y]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Syrian hamster Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    120 µg
    Treatment
    High fat high cholesterol diet +(treatment)
    Specification
    Liver
    Blocking step
    Blocking buffer(10 mM Tris-HCl, 0.9% NaCl, 0.1% Triton X-100, 0.25% gelatin, 0.02% SDS) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 22 2019

    Western blot abreview for Anti-LDL Receptor antibody [EP1553Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chinese hamster Cell lysate - whole cell (Chinese Hamster Ovarian (CHO) cells)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    20 µg
    Specification
    Chinese Hamster Ovarian (CHO) cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 16 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LDL Receptor antibody [EP1553Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 23 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LDL Receptor antibody [EP1553Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 23 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-LDL Receptor antibody [EP1553Y]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (bEnd.3 cell line)
    Permeabilization
    Yes - 0.1% triton
    Specification
    bEnd.3 cell line
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 11 2017

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-LDL Receptor antibody [EP1553Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain Tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    Brain Tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 15 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-LDL Receptor antibody [EP1553Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.1% Tween 20
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 03 2016

    Western blot abreview for Anti-LDL Receptor antibody [EP1553Y]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    5 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Rat Cell lysate - whole cell (liver cells)
    Specification
    liver cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 06 2013

    1-10 of 24 Abreviews or Q&A

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