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RecombinantRabMAb

Recombinant Anti-LDL Receptor antibody [EP1553Y] (ab52818)

  • Datasheet
  • SDS
Reviews (16)Q&A (8)References (82)

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Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
  • Anti-LDL Receptor antibody [EP1553Y] (ab52818)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1553Y] to LDL Receptor
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-LDL Receptor antibody [EP1553Y]
    See all LDL Receptor primary antibodies
  • Description

    Rabbit monoclonal [EP1553Y] to LDL Receptor
  • Host species

    Rabbit
  • Specificity

    Some optimisation may be required for detection of the target protein due to low levels of endogenous expression in some samples.  Please see images below for suitable positive controls.

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human LDL Receptor aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P01130

  • Positive control

    • WB: HeLa and RAW264.7 cell lysate. HepG2 whole cell lysate. Mouse liver, lung and colon lysate. Human liver and plasma lysate. IHC-P: Human hepatocellular carcinoma and liver tissue.
  • General notes

      

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1553Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Second Messenger
    • Lipid Messengers
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • HRP Anti-LDL Receptor antibody [EP1553Y] (ab195516)
    • Anti-LDL Receptor antibody [EP1553Y] - Low endotoxin, Azide free (ab215980)
    • Anti-LDL Receptor antibody [EP1553Y] - BSA and Azide free (ab271846)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human LDL Receptor protein (Active) (ab220570)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocol.

WB (7)
1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
Notes
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocol.

WB
1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
    • Involvement in disease

      Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).
    • Sequence similarities

      Belongs to the LDLR family.
      Contains 3 EGF-like domains.
      Contains 7 LDL-receptor class A domains.
      Contains 6 LDL-receptor class B repeats.
    • Post-translational
      modifications

      N- and O-glycosylated.
      Ubiquitinated by MYLIP leading to degradation.
    • Cellular localization

      Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments.
    • Target information above from: UniProt accession P01130 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3949 Human
      • Entrez Gene: 16835 Mouse
      • Omim: 606945 Human
      • SwissProt: P01130 Human
      • SwissProt: P35951 Mouse
      • Unigene: 213289 Human
      • Unigene: 728190 Human
      • Unigene: 3213 Mouse
      • Alternative names

        • FH antibody
        • FHC antibody
        • LDL R antibody
        • LDL receptor antibody
        • LDLCQ2 antibody
        • Ldlr antibody
        • LDLR_HUMAN antibody
        • Low Density Lipoprotein Receptor antibody
        • Low density lipoprotein receptor class A domain containing protein 3 antibody
        • Low density lipoprotein receptor familial hypercholesterolemia antibody
        • Low-density lipoprotein receptor antibody
        see all

      Images

      • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution

        Lane 1 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
        Lane 2 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
        Lane 3 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
        Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
        Lane 5 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
        Lane 6 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
        Lane 7 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

        Predicted band size: 95 kDa
        Observed band size: 150 kDa why is the actual band size different from the predicted?



        Blocking buffer: 5% NFDM/TBST

        Diluting buffer: 5% NFDM/TBST

      • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution

        Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
        Lane 2 : HeLa treated with GW3965 for 8 hours at the final concentration of 5uM whole cell lysates
        Lane 3 : HeLa treated with GW3965 for 24 hours at the final concentration of 5uM whole cell lysates
        Lane 4 : Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysates
        Lane 5 : Raw264.7 treated with GW3965 for 8 hours at the final concentration of 5uM whole cell lysates
        Lane 6 : Raw264.7 treated with GW3965 for 24 hours at the final concentration of 5uM whole cell lysates

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

        Predicted band size: 95 kDa
        Observed band size: 140 kDa why is the actual band size different from the predicted?


        Exposure time: 1 minute


        Blocking buffer: 5% NFDM/TBST

        Diltuion buffer: 5% NFDM/TBST

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)

        Immunohistochemical analysis of paraffin-embedded human liver sections labeling LDL Receptor with purified ab52818 at dilution of 1:500. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

      • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 0.4 µg/ml + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 95 kDa


        Exposure time: 3 minutes


        Blocking and diluting buffer: 5% NFDM/TBST.

        The molecular weight observed is consistent with the literature (PMID: 15199428, PMID: 8349823, PMID: 10906332, PMID: 24918045).

      • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution

        Lane 1 : Mouse liver lysate
        Lane 2 : Mouse lung lysate
        Lane 3 : Mouse colon lysate
        Lane 4 : Human liver lysate
        Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
        Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (HRP goat anti-rabbit IgG (H+L))

        Predicted band size: 95 kDa
        Observed band size: 140 kDa why is the actual band size different from the predicted?


        Exposure time: 3 minutes


        Blocking buffer: 5% NFDM/TBST

        Diltuion buffer: 5% NFDM/TBST

      • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/5000 dilution + Mouse liver at 15 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

        Predicted band size: 95 kDa
        Observed band size: 140 kDa why is the actual band size different from the predicted?



        Blocking/Diluting buffer 5% NFDM/TBST
      • Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/500 dilution + Human plasma total protein lysate at 10 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 95 kDa
        Observed band size: 100 kDa why is the actual band size different from the predicted?
        Additional bands at: 27 kDa, 48 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 4 minutes


        LDL Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)

        Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling LDL Receptor with ab52818 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counterstained with hematoxylin.

      • Anti-LDL Receptor antibody [EP1553Y] (ab52818)
        Anti-LDL Receptor antibody [EP1553Y] (ab52818)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (82)

      Publishing research using ab52818? Please let us know so that we can cite the reference in this datasheet.

      ab52818 has been referenced in 82 publications.

      • Han M  et al. Therapeutic implications of altered cholesterol homeostasis mediated by loss of CYP46A1 in human glioblastoma. EMBO Mol Med 12:e10924 (2020). PubMed: 31777202
      • Ren Z  et al. Anti-glycolipid disorder effect of epigallocatechin-3-gallate on high-fat diet and STZ-induced T2DM in mice. Mol Med Rep 21:2475-2483 (2020). PubMed: 32236613
      • Tousignant KD  et al. Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancer. Cancer Metab 8:11 (2020). PubMed: 32577235
      • Li T  et al. The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRa and PPARa. J Cell Mol Med 24:3384-3398 (2020). PubMed: 31981312
      • Zhang X  et al. Hydroxytyrosol Plays Antiatherosclerotic Effects through Regulating Lipid Metabolism via Inhibiting the p38 Signal Pathway. Biomed Res Int 2020:5036572 (2020). PubMed: 32685494
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review

      Filter by Application

      Filter by Species

      Filter by Ratings

      1-10 of 16 Abreviews

      Western blot abreview for Anti-LDL Receptor antibody [EP1553Y]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Dopaminergic Neurons & HEK-293T)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      30 µg
      Specification
      Dopaminergic Neurons & HEK-293T
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 16 2020

      Western blot abreview for Anti-LDL Receptor antibody [EP1553Y]

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (kidney)
      Gel Running Conditions
      Reduced Denaturing (4-12% bis-tris gel)
      Loading amount
      30 µg
      Specification
      kidney
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 03 2020

      Western blot abreview for Anti-LDL Receptor antibody [EP1553Y]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Syrian hamster Tissue lysate - whole (Liver)
      Gel Running Conditions
      Reduced Denaturing (10% gel)
      Loading amount
      120 µg
      Treatment
      High fat high cholesterol diet +(treatment)
      Specification
      Liver
      Blocking step
      Blocking buffer(10 mM Tris-HCl, 0.9% NaCl, 0.1% Triton X-100, 0.25% gelatin, 0.02% SDS) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 22°C
      Read More

      Abcam user community

      Verified customer

      Submitted May 22 2019

      Western blot abreview for Anti-LDL Receptor antibody [EP1553Y]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Chinese hamster Cell lysate - whole cell (Chinese Hamster Ovarian (CHO) cells)
      Gel Running Conditions
      Reduced Denaturing (8%)
      Loading amount
      20 µg
      Specification
      Chinese Hamster Ovarian (CHO) cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 16 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LDL Receptor antibody [EP1553Y]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Liver)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
      Permeabilization
      No
      Specification
      Liver
      Blocking step
      PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted May 23 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LDL Receptor antibody [EP1553Y]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Liver)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
      Permeabilization
      No
      Specification
      Liver
      Blocking step
      PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted May 23 2018

      Immunocytochemistry/ Immunofluorescence abreview for Anti-LDL Receptor antibody [EP1553Y]

      Excellent
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (bEnd.3 cell line)
      Permeabilization
      Yes - 0.1% triton
      Specification
      bEnd.3 cell line
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 11 2017

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-LDL Receptor antibody [EP1553Y]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Mouse Tissue sections (Brain Tissue)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Buffer
      Permeabilization
      Yes - 0.2% Triton X-100
      Specification
      Brain Tissue
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 15 2017

      Immunohistochemistry (Frozen sections) abreview for Anti-LDL Receptor antibody [EP1553Y]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Brain)
      Permeabilization
      Yes - 0.1% Tween 20
      Specification
      Brain
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 03 2016

      Western blot abreview for Anti-LDL Receptor antibody [EP1553Y]

      Good
      Abreviews
      Abreviews
      Application
      Western blot
      Loading amount
      5 µg
      Gel Running Conditions
      Reduced Denaturing (8%)
      Sample
      Rat Cell lysate - whole cell (liver cells)
      Specification
      liver cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 06 2013

      1-10 of 16 Abreviews

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