For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Thank you for your prompt reply. I do have one more clarification.
I do not have the chemicals required for preparing Optiblot LDS sample buffer. So can I use the Laemli sample buffer instead? It contains tris HCl buffer pH 6.8, 2% SDS, mercaptoethanol and bromophenol blue.
Asked on Aug 01 2012
Thank you for your reply.
I'd suggest using the sample buffer that is compatible with the gel, namely ab119196.
This is what is recommended to be used.
I can doublecheck this with the lab, but I don't think the standard Laemmli buffer would give you good results. I'll let you know.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
Answered on Aug 01 2012