Key features and details
- Rabbit polyclonal to LEAP2
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-LEAP2 antibody
DescriptionRabbit polyclonal to LEAP2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Cow, Rhesus monkey
- WB: Pig liver and skeletal muscle tissues; Recombinant human LEAP2 protein containing a GST tag. IHC-P: Human prostate gland and rectum tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.
Predicted band: 9 kDa
FunctionHas an antimicrobial activity.
Sequence similaritiesBelongs to the LEAP2 family.
- Information by UniProt
- LEAP-2 antibody
- LEAP2 antibody
- LEAP2_HUMAN antibody
- Liver-expressed antimicrobial peptide 2 antibody
Formalin-fixed, paraffin-embedded human rectum tissue stained for LEAP2 using ab231955 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Anti-LEAP2 antibody (ab231955) at 2 µg/ml + Recombinant human LEAP2 protein containing a GST tag
Observed band size: 39 kDa why is the actual band size different from the predicted?
Formalin-fixed, paraffin-embedded human prostate gland tissue stained for LEAP2 using ab231955 at 10 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-LEAP2 antibody (ab231955) at 3 µg/ml
Lane 1 : Pig liver tissue lysate
Lane 2 : Pig skeletal muscle tissue lysate
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231955 has not yet been referenced specifically in any publications.