Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4452(2)] to LEC
- Suitable for: WB
- Reacts with: Human
Product nameAnti-LEC antibody [EPR4452(2)]
See all LEC primary antibodies
DescriptionRabbit monoclonal [EPR4452(2)] to LEC
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide, corresponding to residues in Human LEC (UniProt ID: O15467)
- Recombinant human LEC protein (ab116400) can be used as a positive control in WB. Human LEC recombinant protein.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.51 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 11 kDa (predicted molecular weight: 14 kDa).|
FunctionShows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.
Tissue specificityMainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma-activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
- Information by UniProt
- C-C motif chemokine 16 antibody
- CCL16 antibody
- CCL16_HUMAN antibody
Anti-LEC antibody [EPR4452(2)] (ab134917) at 1/10000 dilution + Human LEC recombinant protein (10ng)
Secondary Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 14 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab134917 has not yet been referenced specifically in any publications.