Key features and details
- Mouse monoclonal [OTI2A11] to LECT2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-LECT2 antibody [OTI2A11]
See all LECT2 primary antibodies
DescriptionMouse monoclonal [OTI2A11] to LECT2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human LECT2 aa 19-151. Produced in E.coli (NP_002293). aa 1-18 are the signal peptide, immunogen is to the full length mature chain.
GPWANICAGKSSNEIRTCDRHGCGQYSAQRSQRPHQGVDILCSAGSTVYA PFTGMIVGQEKPYQNKNAINNGVRISGRGFCVKMFYIKPIKYKGPIKKGE KLGTLLPLQKVYPGIQSHVHIENCDSSDPTAYL
Database link: O14960
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY LECT2 cDNA. IHC-P: Human breast, Kidney, Lung carcinoma, Ovary adenocarcinoma, Pancreas, Endometrium adenocarcinoma and Tonsil tissues.
The clone number has been updated from 2A11 to OTI2A11, both clone numbers name the same clone.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography
Our Abpromise guarantee covers the use of ab119429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 16 kDa.|
|IHC-P||1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionHas a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation.
Tissue specificityHighly expressed in adult and fetal liver and weakly in testis. Not expressed in bone marrow.
Sequence similaritiesBelongs to the LECT2/MIM-1 family.
Cellular localizationCytoplasm. Secreted.
- Information by UniProt
- chm II antibody
- chm2 antibody
- Chondromodulin II antibody
All lanes : Anti-LECT2 antibody [OTI2A11] (ab119429) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY LECT2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 16 kDa
Paraffin-embedded human breast tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human lung carcinoma tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human ovary adenocarcinoma tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreas tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human endometrium adenocarcinoma tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human tonsil tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
ab119429 has not yet been referenced specifically in any publications.