Key features and details
- Rabbit polyclonal to LEF1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-LEF1 antibody
See all LEF1 primary antibodies
DescriptionRabbit polyclonal to LEF1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- Mouse lymph node and human colon carcinoma lysates; rat liver tissue and human colon carcinoma tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 44 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionParticipates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Tissue specificityDetected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
Sequence similaritiesBelongs to the TCF/LEF family.
Contains 1 HMG box DNA-binding domain.
DomainProline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
Cellular localizationNucleus. Found in nuclear bodies upon PIASG binding.
- Information by UniProt
- DKFZp586H0919 antibody
- FLJ46390 antibody
- LEF 1 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat liver tissue labeling LEF1 with ab217378 at 1/200 dilution followed by secondary detection and DAB staining.
All lanes : Anti-LEF1 antibody (ab217378) at 1/200 dilution
Lane 1 : rat brain lysate
Lane 2 : human colon carcinoma lysate
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Predicted band size: 44 kDa
Anti-LEF1 antibody (ab217378) at 1/300 dilution + Mouse lymph node lysate
Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/500 dilution
Predicted band size: 44 kDa
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human colon carcinoma tissue labeling LEF1 with ab217378 at 1/200 dilution followed by secondary detection and DAB staining.
ab217378 has been referenced in 2 publications.
- Cao L et al. Genotoxic stress-triggered ß-catenin/JDP2/PRMT5 complex facilitates reestablishing glutathione homeostasis. Nat Commun 10:3761 (2019). PubMed: 31434880
- Liu J et al. Stromal fibroblast activation protein alpha promotes gastric cancer progression via epithelial-mesenchymal transition through Wnt/ ß-catenin pathway. BMC Cancer 18:1099 (2018). PubMed: 30419872