Key features and details
- Rabbit polyclonal to LEF1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LEF1 antibody
See all LEF1 primary antibodies
DescriptionRabbit polyclonal to LEF1
Specificityab22884 recognises LEF1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
- Purchase matching WB positive control:Recombinant Human LEF1 protein
- Jurkat cell lysate and human intestine tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab22884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionParticipates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Tissue specificityDetected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
Sequence similaritiesBelongs to the TCF/LEF family.
Contains 1 HMG box DNA-binding domain.
DomainProline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
Cellular localizationNucleus. Found in nuclear bodies upon PIASG binding.
- Information by UniProt
- DKFZp586H0919 antibody
- FLJ46390 antibody
- LEF 1 antibody
Anti-LEF1 antibody (ab22884) at 2 µg/ml + Jurkat cell lysate with 5% skim milk in PBS
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Gel concentration is 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling LEF1 with ab22884 at 4µg/ml. Arows indicate the positively stained intestinal villus epithelial cells.
ab22884 has been referenced in 8 publications.
- Chen C & Shan H Keratin 6A gene silencing suppresses cell invasion and metastasis of nasopharyngeal carcinoma via the ß-catenin cascade. Mol Med Rep 19:3477-3484 (2019). PubMed: 30896882
- Song J et al. Transcription factor AP-4 promotes tumorigenic capability and activates the Wnt/ß-catenin pathway in hepatocellular carcinoma. Theranostics 8:3571-3583 (2018). PubMed: 30026867
- Hughes MW et al. Disrupted ectodermal organ morphogenesis in mice with a conditional histone deacetylase 1, 2 deletion in the epidermis. J Invest Dermatol 134:24-32 (2014). IHC . PubMed: 23792463
- Fancy SP et al. Parallel states of pathological Wnt signaling in neonatal brain injury and colon cancer. Nat Neurosci 17:506-12 (2014). PubMed: 24609463
- Veraitch O et al. Human induced pluripotent stem cell-derived ectodermal precursor cells contribute to hair follicle morphogenesis in vivo. J Invest Dermatol 133:1479-88 (2013). PubMed: 23321923
- Soma T et al. Hair-inducing ability of human dermal papilla cells cultured under Wnt/ß-catenin signalling activation. Exp Dermatol 21:307-9 (2012). WB ; Human . PubMed: 22417309
- Spee B et al. Characterisation of the liver progenitor cell niche in liver diseases: potential involvement of Wnt and Notch signalling. Gut 59:247-57 (2010). IHC-Fr ; Human . PubMed: 19880964
- Yoo BY et al. Application of mesenchymal stem cells derived from bone marrow and umbilical cord in human hair multiplication. J Dermatol Sci 60:74-83 (2010). IHC-P ; Human . PubMed: 20956069