Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab52017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa). The observed molecular weight corresponds to earlier findings in literature (Yasumoto et al , EMBO J. 2002 Jun 3;21(11):2703-14.; PMID: 12032083). Immunofluorescence: This antibody was successfully used to stain LEF1 in Zebrafish fish fins. Fins were fixed with paraformaldehyde at 4°C (See PMID 19015519).
EMSA Use at an assay dependent concentration. PubMed: 22011226

Target

  • Function
    Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
  • Tissue specificity
    Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
  • Sequence similarities
    Belongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.
  • Domain
    Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
  • Cellular localization
    Nucleus. Found in nuclear bodies upon PIASG binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp586H0919 antibody
    • FLJ46390 antibody
    • LEF 1 antibody
    • LEF-1 antibody
    • Lef1 antibody
    • LEF1_HUMAN antibody
    • Lymphoid enhancer binding factor 1 antibody
    • Lymphoid enhancer-binding factor 1 antibody
    • T cell specific transcription factor 1 alpha antibody
    • T cell-specific transcription factor 1-alpha antibody
    • TCF 1 alpha antibody
    • TCF1 alpha antibody
    • TCF1-alpha antibody
    • TCF10 antibody
    • TCF1alpha antibody
    • TCF7L3 antibody
    • Transcription factor T cell specific 1 alpha antibody
    see all

Images

  • Anti-LEF1 antibody (ab52017) at 1 µg/ml + Daudi cell lysate (35µg protein in RIPA buffer).

    Predicted band size: 44 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.

References

This product has been referenced in:
  • Dai Y  et al. Overexpression of parkin protects retinal ganglion cells in experimental glaucoma. Cell Death Dis 9:88 (2018). WB . Read more (PubMed: 29367744) »
  • Wu Y  et al. Long noncoding RNA hypoxia-inducible factor 1 alpha-antisense RNA 1 promotes tumor necrosis factor-a-induced apoptosis through caspase 3 in Kupffer cells. Medicine (Baltimore) 97:e9483 (2018). Read more (PubMed: 29369172) »
See all 4 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting Abcam. I believe that both choices that you have made are excellent. Both are covered by our Abpromise guarantee so you need not be concerned that the product will work for you should you use them in the manner consistant with that stated on the website. We will support and assist you should any issues arise and will replace or refund the antibody if needed. I am hesitant to suggest one product over the other as I feel they will both work well for your needs. My only preference when choosing between these two products is from knowing the exact immunogen sequence used to create ab52017 and the greater number (+1) of publications that have cited it. Please feel free to contact me should you have questions or if there is anything else that I may do to help.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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