Key features and details
- Rabbit polyclonal to LEF1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LEF1 antibody
See all LEF1 primary antibodies
DescriptionRabbit polyclonal to LEF1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Pig, Monkey
Synthetic peptide corresponding to Human LEF1 aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Purchase matching WB positive control:Recombinant Human LEF1 protein
- This antibody gave a positive signal in Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42, 50 kDa (predicted molecular weight: 44 kDa).|
FunctionParticipates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Tissue specificityDetected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
Sequence similaritiesBelongs to the TCF/LEF family.
Contains 1 HMG box DNA-binding domain.
DomainProline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
Cellular localizationNucleus. Found in nuclear bodies upon PIASG binding.
- Information by UniProt
- DKFZp586H0919 antibody
- FLJ46390 antibody
- LEF 1 antibody
Anti-LEF1 antibody (ab74549) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 42 + 50 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
ab74549 detects a band at 42 and 50 kDa. While this differs to its predicted molecular weight of 44 kDa, this migration has been observed in the literature PMID:17335794.
ab74549 has not yet been referenced specifically in any publications.