Recombinant
RabMAb

Anti-LEF1 antibody [EP2030Y] (ab53293)

Abreviews (2)Q&A (3)Specific References (7)

Overview

  • Product name
    Anti-LEF1 antibody [EP2030Y]
    See all LEF1 primary antibodies
  • Description
    Rabbit monoclonal [EP2030Y] to LEF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in human LEF1.

  • Positive control
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab53293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 44 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function
      Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
    • Tissue specificity
      Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
    • Sequence similarities
      Belongs to the TCF/LEF family.
      Contains 1 HMG box DNA-binding domain.
    • Domain
      Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
    • Cellular localization
      Nucleus. Found in nuclear bodies upon PIASG binding.
    • Information by UniProt
    • Database links
      • Alternative names
        • DKFZp586H0919 antibody
        • FLJ46390 antibody
        • LEF 1 antibody
        • LEF-1 antibody
        • Lef1 antibody
        • LEF1_HUMAN antibody
        • Lymphoid enhancer binding factor 1 antibody
        • Lymphoid enhancer-binding factor 1 antibody
        • T cell specific transcription factor 1 alpha antibody
        • T cell-specific transcription factor 1-alpha antibody
        • TCF 1 alpha antibody
        • TCF1 alpha antibody
        • TCF1-alpha antibody
        • TCF10 antibody
        • TCF1alpha antibody
        • TCF7L3 antibody
        • Transcription factor T cell specific 1 alpha antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-LEF1 antibody [EP2030Y] (ab53293)
        Immunocytochemistry/ Immunofluorescence - Anti-LEF1 antibody [EP2030Y] (ab53293)

        ab53293 staining LEF1 in RAMOS (human Burkitt's lymphoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain and the negative control was PBS only.

      • Western blot - Anti-LEF1 antibody [EP2030Y] (ab53293)
        Western blot - Anti-LEF1 antibody [EP2030Y] (ab53293)
        Anti-LEF1 antibody [EP2030Y] (ab53293) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 44 kDa
        Observed band size: 25-57 kDa (why is the actual band size different from the predicted?)


        Exposure time: 1 minute


        Blocking/Diluting buffer 5% NFDM/TBST.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EP2030Y] (ab53293)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EP2030Y] (ab53293)

        Paraffin-embedded human colon adenocarcinoma tissue stained for LEF1 using ab53293 at 1/50 dilution in immunohistochemical analysis.

         

      • Flow Cytometry - Anti-LEF1 antibody [EP2030Y] (ab53293)
        Flow Cytometry - Anti-LEF1 antibody [EP2030Y] (ab53293)

        Flow cytometric analysis of permeabilized Jurkat cells using ab53293 (red) or a rabbit IgG (negative) (green).

      References

      This product has been referenced in:
      • Murakami T  et al. Isolation and characterization of lymphoid enhancer factor-1-positive deciduous dental pulp stem-like cells after transfection with a piggyBac vector containing LEF1 promoter-driven selection markers. Arch Oral Biol 81:110-120 (2017). ICC/IF ; Human . Read more (PubMed: 28500952) »
      • Zhang X  et al. MicroRNA-195 suppresses colorectal cancer cells proliferation via targeting FGF2 and regulating Wnt/ß-catenin pathway. Am J Cancer Res 6:2631-2640 (2016). WB ; Human . Read more (PubMed: 27904776) »

      See all 7 Publications for this product

      Publishing research using ab53293? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      5 reviews or Q&A

      10
      Votes: Highest First

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LEF1 antibody [EP2030Y]

       Average
      Abreviews
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Pig Tissue sections (unerupted tooth buds)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 10mM citric Acid monobasic pH6
      Permeabilization
      No
      Specification
      unerupted tooth buds
      Blocking step
      BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
      Fixative
      10% Formalin
      Read More
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      Username

      Abcam user community

      Verified customer

      Submitted Apr 27 2017

      Dear Sir/Madam,
      I am using the Anti-LEF1 antibody [EP2030Y] (ab53293) for cytometry analysis and would like to know if it recognizes all the known LEF1 isoforms.
      Thank you in advance,

      Thank you for your inquiry.
      The exact immunogen sequence is proprietary information and I unfortunately do not have access to it.
      But the lab confirmed that all isoforms should be recognized by ab53293 since all isoforms contain the immunogen...

      Read More

      Dear Madam / Sir,

      A researher using ab53293 lef-1 has a band 70kDa; but It is written a expected band at app. 55kDa on the datasheet. According to uniport, lef-1 doesn't have any isoform with mass 70 kDa. Do you have any interpretation why I...

      Read More

      Thank you for taking the time to contact us. I am sorry to hearthe customer has some concerns regarding the results from this antibody.

      I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB and...

      Read More

      I use the primary antibody(cat:ab53293) to detect lung fibroblast lef1 content in western blot. But I can't find any band whatever the concentration I used. I ask whether you have positive control sample? If you have, please send it to me.

      Thank you for contacting Abcam.

      I am sorry that you are having problems with ab53293 in western blot. If you are looking for a positive control then I would recommend ab7899:

      https://www.abcam.com/Jurkat-Human-T-cell-lymphoblast-lik...

      Read More

      Flow Cytometry abreview for Anti-LEF1 antibody [EP2030Y]

       Good
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Flow Cytometry
      Sample
      Human Cell (REH B cell)
      Specification
      REH B cell
      Fixation
      Paraformaldehyde
      Permeabilization
      Yes - saponin
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted Apr 01 2010

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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