Anti-LEF1 antibody [EP2030Y] (ab53293)
- Datasheet
- Specific References (7)
- Protocols
Overview
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Product nameAnti-LEF1 antibody [EP2030Y]
See all LEF1 primary antibodies -
DescriptionRabbit monoclonal [EP2030Y] to LEF1
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
Unsuitable for: IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide corresponding to residues in human LEF1.
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Positive control
- Purchase matching WB positive control:Recombinant Human LEF1 protein
- ICC/IF: Ramos cells. WB: Jurkat whole cell lysate. IHC-P: Human colon adenocarcinoma. Flow Cyt: Jurkat cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP2030Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab53293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 44 kDa). | |
| Flow Cyt | 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionParticipates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
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Tissue specificityDetected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
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Sequence similaritiesBelongs to the TCF/LEF family.
Contains 1 HMG box DNA-binding domain. -
DomainProline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
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Cellular localizationNucleus. Found in nuclear bodies upon PIASG binding.
- Information by UniProt
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Database links
- Entrez Gene: 51176 Human
- Omim: 153245 Human
- SwissProt: Q9UJU2 Human
- Unigene: 726506 Human
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Alternative names
- DKFZp586H0919 antibody
- FLJ46390 antibody
- LEF 1 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-LEF1 antibody [EP2030Y] (ab53293)](https://www.abcam.com/ps/products/53/ab53293/Images/ab53293-263746-ab53293-Ramos-500.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LEF1 antibody [EP2030Y] (ab53293)ab53293 staining LEF1 in RAMOS (human Burkitt's lymphoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain and the negative control was PBS only.
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![Western blot - Anti-LEF1 antibody [EP2030Y] (ab53293)](https://www.abcam.com/ps/products/53/ab53293/Images/ab53293-261863-ab53293WB.jpg)
Western blot - Anti-LEF1 antibody [EP2030Y] (ab53293)Anti-LEF1 antibody [EP2030Y] (ab53293) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 25-57 kDa (why is the actual band size different from the predicted?)
Exposure time: 1 minuteBlocking/Diluting buffer 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EP2030Y] (ab53293)](https://www.abcam.com/ps/products/53/ab53293/Images/ab53293_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EP2030Y] (ab53293)Paraffin-embedded human colon adenocarcinoma tissue stained for LEF1 using ab53293 at 1/50 dilution in immunohistochemical analysis.
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![Flow Cytometry - Anti-LEF1 antibody [EP2030Y] (ab53293)](https://www.abcam.com/ps/products/53/ab53293/Images/ab53293_3.jpg)
Flow Cytometry - Anti-LEF1 antibody [EP2030Y] (ab53293)Flow cytometric analysis of permeabilized Jurkat cells using ab53293 (red) or a rabbit IgG (negative) (green).
References
This product has been referenced in:
- Murakami T et al. Isolation and characterization of lymphoid enhancer factor-1-positive deciduous dental pulp stem-like cells after transfection with a piggyBac vector containing LEF1 promoter-driven selection markers. Arch Oral Biol 81:110-120 (2017). ICC/IF ; Human . Read more (PubMed: 28500952) »
- Zhang X et al. MicroRNA-195 suppresses colorectal cancer cells proliferation via targeting FGF2 and regulating Wnt/ß-catenin pathway. Am J Cancer Res 6:2631-2640 (2016). WB ; Human . Read more (PubMed: 27904776) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"