Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (unerupted tooth buds)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citric Acid monobasic pH6
Permeabilization
No
Specification
unerupted tooth buds
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
10% Formalin
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA
Dilution
1/100
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: goat ant Rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
The lowest concentrated we tested was 1:100 which allows for very low detection of Lef-1 in pig unerupted tooth bud paraffin sections. A higher concentration may work better on similar sections. SCALE BAR: 50um.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Apr 27 2017