Anti-LEF1 antibody [EPR2029Y] - BSA and Azide free (ab215999)
- Datasheet
- References (2)
- Protocols
Overview
-
Product nameAnti-LEF1 antibody [EPR2029Y] - BSA and Azide free
See all LEF1 primary antibodies -
DescriptionRabbit monoclonal [EPR2029Y] to LEF1 - BSA and Azide free
-
Host speciesRabbit
-
Tested applicationsSuitable for: IP, Flow Cyt, IHC-FoFr, ICC/IF, IHC-P, WBmore details
-
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide corresponding to residues in Human LEF1.
-
Positive control
- WB: Jurkat cell lysate. IHC-P: Human tonsil tissue.
-
General notes
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferConstituent: PBS
-
Concentration information loading... -
PurityProtein A purified
-
ClonalityMonoclonal
-
Clone numberEPR2029Y
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab215999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 24586192 | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 44 kDa. |
Target
-
FunctionParticipates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
-
Tissue specificityDetected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
-
Sequence similaritiesBelongs to the TCF/LEF family.
Contains 1 HMG box DNA-binding domain. -
DomainProline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
-
Cellular localizationNucleus. Found in nuclear bodies upon PIASG binding.
- Information by UniProt
-
Database links
- Entrez Gene: 51176 Human
- Entrez Gene: 16842 Mouse
- Entrez Gene: 161452 Rat
- Omim: 153245 Human
- SwissProt: Q9UJU2 Human
- SwissProt: P27782 Mouse
- SwissProt: Q9QXN1 Rat
- Unigene: 726506 Human
see all -
Alternative names
- DKFZp586H0919 antibody
- FLJ46390 antibody
- LEF 1 antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] - BSA and Azide free (ab215999)](https://www.abcam.com/ps/products/215/ab215999/Images/ab215999-291605-ab137872IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] - BSA and Azide free (ab215999)This IHC data was generated using the same anti-LEF1 antibody clone, EPR2029Y, in a different buffer formulation (cat# ab137872).
Ab137872 staining LEF1 in paraffin embedded Mouse spleen tissue by Immunohistochemistry. Antigen retrieval was by heat mediation using ab93684 (Tris/EDTA buffer, Ph9). Samples were incubated with primary antibody at 1:2000 dilution. A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counter stain. Nuclear staining on T cells in periarterial lymphatic sheath of mouse spleen (PMID: 21685909).
Datasheets and documents
Protocols
References
This product has been referenced in:
- Skokowa J et al. Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for G-CSF-triggered granulopoiesis. Nat Med 18:1550-9 (2012). Read more (PubMed: 23001182) »
- Tandon B et al. Nuclear overexpression of lymphoid-enhancer-binding factor 1 identifies chronic lymphocytic leukemia/small lymphocytic lymphoma in small B-cell lymphomas. Mod Pathol 24:1433-43 (2011). IHC ; Human . Read more (PubMed: 21685909) »
