Key features and details
- Mouse monoclonal [7C5G1H6H10] to Lefty
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-Lefty antibody [7C5G1H6H10]
See all Lefty primary antibodies
DescriptionMouse monoclonal [7C5G1H6H10] to Lefty
SpecificityThis antibody reacts with a 30 kDa protein corresponding to human Lefty. No reactivity occurs against 6X HIS tag.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant 6X HIS tagged human Lefty.
General notesHybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab34593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 41 kDa).|
RelevanceDuring vertebrate embryogenesis, a left right axis is established. Secreted growth factors of the TGF beta family, including gene products derived from nodal, lefty 1 and lefty 2, play crucial roles in establishing left right asymmetries. TGF beta (Transforming growth factor beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF beta actions are directed by ligand induced activation of TGF beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF beta superfamily, inhibits TGF beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF beta receptor. Lefty also inhibits events downstream from R Smad phosphorylation. Lefty provides a repressed state of TGF beta responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (41 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
- AI450052 antibody
- Ebaf antibody
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All lanes : Anti-Lefty antibody [7C5G1H6H10] (ab34593) at 1/2000 dilution
Lane 1 : 1µg 6X His tagged unrelated protein (shows that the antibody does not recognize the epitope tag).
Lane 2 : 1µg partially purified recombinant 6X his tagged human Lefty
All lanes : IRDyeTM800 Goat anti-mouse IgG at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
ab34593 has not yet been referenced specifically in any publications.