• Product name
  • Description
    Rabbit polyclonal to Lefty
  • Host species
  • Specificity
    This antibody has been tested against recombinant protein only (human LeftyA and mouse Lefty1). I is expected to cross react with Human LeftyB as there is 100% homology of the immunogen with Human LeftyB.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Lefty.

    Read Abcam's proprietary immunogen policy (Peptide available as ab25701.)

  • Positive control
    • recombinant Lefty1/ LeftyA


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab22569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at a concentration of 1 µg/ml. Detects a bands of approximately 44, 38 and 34 kDa (predicted molecular weight: 41 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      During vertebrate embryogenesis, a left right axis is established. Secreted growth factors of the TGF beta family, including gene products derived from nodal, lefty 1 and lefty 2, play crucial roles in establishing left right asymmetries. TGF beta (Transforming growth factor beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF beta actions are directed by ligand induced activation of TGF beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF beta superfamily, inhibits TGF beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF beta receptor. Lefty also inhibits events downstream from R Smad phosphorylation. Lefty provides a repressed state of TGF beta responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (41 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
    • Cellular localization
    • Database links
    • Alternative names
      • AI450052 antibody
      • Ebaf antibody
      • Left right determination factor 1 antibody
      • Left right determination factor antibody
      • Left right determination factor B antibody
      • left-right determination factor antibody
      • left-right determination, factor B antibody
      • LEFTB antibody
      • LEFTY 1 antibody
      • Lefty antibody
      • lefty-1 antibody
      • LEFTY1 antibody
      • LEFTY1, MOUSE, HOMOLOG OF antibody
      • LEFTYB antibody
      • OTTHUMP00000035572 antibody
      • Protein lefty-1 antibody
      • Protein lefty-B antibody
      • RGD1561867 antibody
      • Stimulated by retinoic acid gene 3 protein antibody
      • STRA3 antibody
      • TGF beta 4 antibody
      • TGF-beta-4 antibody
      • Tgfb4 antibody
      • Transforming growth factor beta 4 antibody
      • Transforming growth factor beta-4 antibody
      see all


    • ICC/IF image of ab22569 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22569, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
    • All lanes : Anti-Lefty antibody (ab22569) at 1 µg/ml

      Lane 1 : Mouse Lefty1 Recombinant Protein
      Lane 2 : Human LeftyA Recombinant Protein

      Lysates/proteins at 0.01 µg per lane.

      All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

      Predicted band size: 41 kDa
      Observed band size: 34,38,44 kDa
      why is the actual band size different from the predicted?

      The mouse recombinant Lefty protein consists of two bands of ~34 and 12 kDa. Of these, ab22569 recognises the 34 kDa band. The human recombinant Lefty protein consists of three bands of 44, 38 and 34 kDa. Of these, the first two are recognised by ab22569. The protein sizes differ from the expected molecular weight of Lefty due to proteolytic processing and glycosylation.


    This product has been referenced in:
    • Xu C  et al. Lefty1 alleviates renal tubulointerstitial injury in mice with unilateral ureteral obstruction. Mol Med Rep 13:901-8 (2016). IHC-P ; Mouse . Read more (PubMed: 26647776) »
    • Yoon SJ  et al. HEB associates with PRC2 and SMAD2/3 to regulate developmental fates. Nat Commun 6:6546 (2015). WB, IP ; Mouse . Read more (PubMed: 25775035) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Western blot
    Human Cell lysate - whole cell (Embryonic Stem Cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Embryonic Stem Cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Jianlong Wang

    Verified customer

    Submitted Apr 23 2018


    Vielen Dank für Ihr Interesse an unseren Produkten.

    Wir haben diese beiden Antikörper leider noch gar nicht an endogenen Proben getestet, so dass wir leider keine Aussage treffen können, welcher besser mit Mausproben funktioniert.

    Da das Immunogen in beiden Fällen mit dem C-Terminus des Proteins korrespondiert, sollte es das unreife Vollprotein AS 1-368 und die reife Form 77-368 erkennen, allerdings nicht das einzelne Signalpeptide oder das Propeptid Or 135.

    Ich habe mir unsere Produkte für dieses Protein einmal angesehen, und es sieht so aus, dass fast alle bisher nur am rekombinanten Protein getetstetwurden.

    Falls Sie einen oder beide Antikörper an dem endogenen Protein selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab22569 und/oder ab30955 abdecken, und gegen eine erneute Bestellung von uns verrechnet werden.

    Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

    1.) Bestätigen Sie mir bitte, dass Sie ab22569 und/oder ab30955 am endogenen Protein testen möchten.

    2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

    3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

    4.) Testen Sie den/die Antikörper in endogenen Proteinproben.

    5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
    Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:

    6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv. Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

    Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

    Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:

    Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Mouse Tissue sections (E6.5 embryo in decidua)
    E6.5 embryo in decidua
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric Acid
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

    Dr. Jesse Mager

    Verified customer

    Submitted Feb 13 2009


    Thank you for your enquiry regarding ab22569. This antibody will recognise human lefty1 and left2 according to a BLAST search with the immunogen sequence though it has only been tested against recombinant Lefty1. Regarding its cross reactivity to mouse protein, it has only been tested against recombinant Lefty1 and not Lefty2. The immunogen has 88% homology with mouse left2 so it may recognise Lefty2 but as this has not been tested we do not know for certain. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More


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