Recombinant
RabMAb

Recombinant Anti-LEFTY1 + LEFTY2 antibody [EPR5444] (ab204283)

Overview

  • Product name

    Anti-LEFTY1 + LEFTY2 antibody [EPR5444]
    See all LEFTY1 + LEFTY2 primary antibodies
  • Description

    Rabbit monoclonal [EPR5444] to LEFTY1 + LEFTY2
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes LEFTY1 and LEFTY2.
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human LEFTY1 + LEFTY2 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary. Also SwissProt ID: O75610
    Database link: O00292

  • Positive control

    • HeLa, 293T and Caco 2 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.90 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5444
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab204283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 34, 44 kDa (predicted molecular weight: 41 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Relevance

      Function for LEFTY1: Required for left-right axis determination as a regulator of LEFTY2 and NODAL. Function for LEFTY2: Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding. Post-translational modification: The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner. Sequence similarities: Belongs to the TGF-beta family. Subcellular location: Secreted. Expression for LEFTY2: Tissue specificity : Mesenchymal cells of the endometrial stroma. Developmental stage: Transiently expressed before and during menstrual bleeding.
    • Database links

    • Alternative names

      • EBAF antibody
      • Endometrial bleeding-associated factor antibody
      • Left-right determination factor 1 antibody
      • Left-right determination factor 2 antibody
      • Left-right determination factor A antibody
      • Left-right determination factor B antibody
      • LEFTA antibody
      • LEFTB antibody
      • LEFTY1 antibody
      • LEFTYA antibody
      • LEFTYB antibody
      • Protein lefty-1 antibody
      • Protein lefty-2 antibody
      • Protein lefty-A antibody
      • Protein lefty-B antibody
      • PSEC0024 antibody
      • TGF-beta-4 antibody
      • TGFB4 antibody
      • Transforming growth factor beta-4 antibody
      • UNQ278/PRO317 antibody
      see all

    Images

    • All lanes : Anti-LEFTY1 + LEFTY2 antibody [EPR5444] (ab204283) at 1/10000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : 293T cell lysates
      Lane 3 : Caco 2 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 41 kDa
      Observed band size: 34,44 kDa
      why is the actual band size different from the predicted?



      The 34kDa band represents mature LEFTY1 + LEFTY2.

    • Equilibrium disassociation constant (KD)

      Learn more about KD


      Click here to learn more about KD

       

    References

    This product has been referenced in:

    • Gao X  et al. miR-215 promotes epithelial to mesenchymal transition and proliferation by regulating LEFTY2 in endometrial cancer. Int J Mol Med 42:1229-1236 (2018). Read more (PubMed: 29845221) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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