Product nameAnti-LEFTY1 + LEFTY2 antibody [EPR5444]
See all LEFTY1 + LEFTY2 primary antibodies
DescriptionRabbit monoclonal [EPR5444] to LEFTY1 + LEFTY2
SpecificityThis antibody recognizes LEFTY1 and LEFTY2.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human LEFTY1 + LEFTY2 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary. Also SwissProt ID: O75610
Database link: O00292
- HeLa, 293T and Caco 2 cell lysates.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.90 x 10 -12 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab204283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 34, 44 kDa (predicted molecular weight: 41 kDa).|
|IP||1/10 - 1/100.|
RelevanceFunction for LEFTY1: Required for left-right axis determination as a regulator of LEFTY2 and NODAL. Function for LEFTY2: Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding. Post-translational modification: The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner. Sequence similarities: Belongs to the TGF-beta family. Subcellular location: Secreted. Expression for LEFTY2: Tissue specificity : Mesenchymal cells of the endometrial stroma. Developmental stage: Transiently expressed before and during menstrual bleeding.
- EBAF antibody
- Endometrial bleeding-associated factor antibody
- Left-right determination factor 1 antibody
All lanes : Anti-LEFTY1 + LEFTY2 antibody [EPR5444] (ab204283) at 1/10000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : 293T cell lysates
Lane 3 : Caco 2 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 34,44 kDa why is the actual band size different from the predicted?
The 34kDa band represents mature LEFTY1 + LEFTY2.
Equilibrium disassociation constant (KD)
Learn more about KD