Key features and details
- Rabbit polyclonal to LEFTY2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LEFTY2 antibody
See all LEFTY2 primary antibodies
DescriptionRabbit polyclonal to LEFTY2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human LEFTY2 aa 57-366.
VRAQYVVLLRRSHGDRSRGKRFSQSFREVAGRFLASEASTHLLVFGMEQR LPPNSELVQAVLRLFQEPVPKAALHRHGRLSPRSAQARVTVEWLRVRDDG SNRTSLIDSRLVSVHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQRE HLGPLASGAHKLVRFASQGAPAGLGEPQLELHTLDLRDYGAQGDCDPEAP MTEGTRCCRQEMYIDLQGMKWAKNWVLEPPGFLAYECVGTCQQPPEALAF NWPFLGPRQCIASETASLPMIVSIKEGGRTRPQVVSLPNMRVQKCSCASD GALVPRRLQP
Database link: O00292
- WB: A431 whole cell lysate. IHC-P: Human endometrial and colon cancer tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 41 kDa (predicted molecular weight: 41, 37 kDa).|
FunctionRequired for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding.
Tissue specificityMesenchymal cells of the endometrial stroma.
Involvement in diseaseDefects in LEFTY2 are the cause of left-right axis malformations (LRAM) [MIM:601877]. The defect includes left pulmonary isomerism, with cardiac anomalies characterized by complete atrioventricular canal defect and hypoplastic left ventricle, and interrupted inferior vena cava.
Sequence similaritiesBelongs to the TGF-beta family.
Developmental stageTransiently expressed before and during menstrual bleeding.
modificationsThe processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.
- Information by UniProt
- EBAF antibody
- Endometrial bleeding associated factor (left right determination factor A transforming growth factor beta superfamily) antibody
- Endometrial bleeding associated factor antibody
Anti-LEFTY2 antibody (ab229668) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate
Goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 41, 37 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
Paraffin-embedded human endometrial cancer tissue stained for LEFTY2 using ab229668 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human colon cancer tissue stained for LEFTY2 using ab229668 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229668 has been referenced in 1 publication.
- Gao X et al. miR-215 promotes epithelial to mesenchymal transition and proliferation by regulating LEFTY2 in endometrial cancer. Int J Mol Med 42:1229-1236 (2018). PubMed: 29845221