Anti-Legionella pneumophila antibody (ab20943)

Reviews (1)Q&A (3)References (2)

Key features and details

  • Rabbit polyclonal to Legionella pneumophila
  • Suitable for: ELISA, ICC/IF
  • Isotype: IgG

Overview

  • Product name

    Anti-Legionella pneumophila antibody
    See all Legionella pneumophila primary antibodies

  • Description

    Rabbit polyclonal to Legionella pneumophila

  • Host species

    Rabbit

  • Specificity

    Reacts with L. pneumophila serogroups 1-12 in IFA. Recognizes all antigens of intact microorganism. Antiserum is not absorbed and may cross-react with related microorganisms

  • Tested applications

    Suitable for: ELISA, ICC/IFmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    A whole cell preparation of Legionella pneumophila; ATCC #33152.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    Legionella pneumophila is a flagellated gram negative bacterium found primarily in warm water environments. Legionella pneumophila causes a type of pneumonia called Legionnaire disease and a milder condition called Pontiac fever. Infection is acquired through inhalation of the microorganism in water mists (e.g. from air conditioning, cooling towers, whirlpool spas and showers mists). A number of risk factors for acquiring Legionnaire disease have been identified, including age, smoking, chronic lung disease, cancer, and immunosuppression.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab20943? Please let us know so that we can cite the reference in this datasheet.

ab20943 has been referenced in 2 publications.

  • Shin D  et al. Regulation of Phosphoribosyl-Linked Serine Ubiquitination by Deubiquitinases DupA and DupB. Mol Cell 77:164-179.e6 (2020). PubMed: 31732457
  • Arambula D  et al. Surface display of a massively variable lipoprotein by a Legionella diversity-generating retroelement. Proc Natl Acad Sci U S A 110:8212-7 (2013). WB . PubMed: 23633572

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Legionella pneumophila antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Legionella bozemanae Cell (Legionella bozenanae clinical strain)
Permeabilization
Yes - 0.1% Triton-X Solution
Specification
Legionella bozenanae clinical strain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Mrs. Breanne Head

Verified customer

Submitted May 20 2019

Question

thank you very much for your info.

About anti Legionella, the customer would be interested in particular in anti Legionella spp and not to Legionella pneumophila.

Would you be able to let me know if your Ab anti Legionella pneumophila can react also with other species of Legionella?

If so, can you please provide us with some data?



Thanks in advance and kind regards

Read More
Answer

Thank you for contacting us.

We do not have antibody specific to Legionella species which can detect all the legionella organnisms however the antibody ab20943, is not a absorbed product so it may cross-react with related microorganisms.

I hope this information will be helpful. Should you have any other question please do not hesitate to hesitate to contact us.

Read More

Question

Dear All,
we have a customer interested in studying the following targets:
- Aspergillus
Legionella pneumophila
Pseudomonas spp
She is looking for Abs against these targets and with the following characteristics:
- If available polyclonal as first choice, or monoclonal
- NOT conjugated
Obtained by immunizing live cells / native organism, or with membrane preparations or (no soluble extracts for example)
- Able to recognize the whole organism native / vital
I did a preliminary check in your catalogue and found the following Abs (please see attachment).
Could you please confirm in particular that these Abs are able to recognize the whole and vital organisme.
If these Abs are not able to recognize the whole and vital organisme can you please check if you have monoclonal Abs with these characteristics?
Thanks in advance for your precious support.
Kind regards

Read More
Answer

Thank you for contacting us.

The following antibodies matches customer request. Please find attached the links;

https://www.abcam.com/legionella-pneumophila-antibody-ab20943.html

https://www.abcam.com/legionella-pneumophila-antibody-ab65287.html

https://www.abcam.com/pseudomonas-antibody-ab68538.html

We do not have anti Aspergillus antibodies as per customer request.

Please suggest customer to check the datasheets.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

Inquiry: Do you know if I can use this product "Anti-Legionella pneumophila antibody (ab20943)" for immunoprecipitation??? DO you know if this product don't react against L.pneumophila sg. 13,14,15 or you haven't tested it?

Read More
Answer

Thank you very much for your interest in ab20943.

ab20943 is tested and guaranteed for L. pneumophila serogroups 1-12. Serogroups 13-15 have not been tested and we do not know if this antibody will recognize them.

To our knowledge, ab20943 has also not been tested in IP. Therefore, I can offer a discount off a future purchase if you buy ab20943 now, test it in IP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of ab20943 off a future order.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab20943 in IP. I will then send a discount code. This code must be issued before purchasing ab20943 so please wait for my reply before ordering.

2. Purchase ab20943 either by phone, fax, or online (www.abcam.com).

3. Test it in IP.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab20943 turns out to be unsuitable for IP, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com