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    legionella-pneumophila-igm-elisa-kit-ab247198.pdf

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Microbiology Organism Bacteria Gram negative Bacteria Legionella
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Legionella Pneumophila IgM ELISA kit (ab247198)

  • Datasheet
  • SDS
  • Protocol Booklet
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Serologic ELISA assay principle

    Key features and details

    • Sample type: Cit plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (qualitative)
    • Reacts with: Human

    Overview

    • Product name

      Legionella Pneumophila IgM ELISA kit
      See all Legionella pneumophila kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      sample 24 0.461nM 4.23%
      sample 24 1.003nM 2.12%
      sample 24 0.862nM 2.65%
      Inter-assay
      Sample n Mean SD CV%
      sample 12 21.35nM 5.1%
      sample 12 15.46nM 7.62%
      sample 12 4.22nM 11.86%
    • Sample type

      Serum, Hep Plasma, Cit plasma
    • Assay type

      Sandwich (qualitative)
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Legionella pneumophila IgM ELISA kit (ab247198) is designed for the qualitative determination of IgM class antibodies against Legionella pneumophila in human serum or plasma (citrate, heparin).


      The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.

    • Notes

      Legionellae are aerobic gram-negative facultative intracellular parasites of certain protozoa. They are found in freshwater environments worldwide and can cause respiratory disease (legionellosis) in humans. L. pneumophila multiplies itself at temperatures between 25 and 42 °C, with an optimal growth temperature of 35 °C. Legionella thrives in warm, stagnant water in the environment and in artificial systems such as cooling towers, evaporative condensers, hot and cold water systems and spa pools that mimic the natural environment in which the organism thrives. These systems also provide the means by which aerosols/droplets are generated and the organism dispersed into the atmosphere.

      Legionellosis can be acquired by the inhalation of aerosols containing Legionella bacteria or by micro-aspiration of ingested water contaminated with Legionella. Person-to-person transmission is not thought to be a risk.

    • Platform

      Microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      20X Washing Solution White cap 1 x 50ml
      anti-human IgM HRP conjugate 1 x 20ml
      Cover Foil 1 unit
      IgM Cut-off Control 1 x 3ml
      IgM Negative Control 1 x 2ml
      IgM Positive Control 1 x 2ml
      IgM Sample Diluent 1 x 100ml
      Legionella pneumophila Coated Microplate (IgM) 1 unit
      Stop Solution red cap 1 x 15ml
      TMB Substrate Solution Yellow cap 1 x 15ml
    • Research areas

      • Microbiology
      • Organism
      • Bacteria
      • Gram negative Bacteria
      • Legionella
    • Relevance

      Legionella pneumophila is a flagellated gram negative bacterium found primarily in warm water environments. Legionella pneumophila causes a type of pneumonia called Legionnaire disease and a milder condition called Pontiac fever. Infection is acquired through inhalation of the microorganism in water mists (e.g. from air conditioning, cooling towers, whirlpool spas and showers mists). A number of risk factors for acquiring Legionnaire disease have been identified, including age, smoking, chronic lung disease, cancer, and immunosuppression.
    • Database links

      • Omim: 608556 Human

      Images

      • Serologic ELISA assay principle
        Serologic ELISA assay principle
        Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab247198? Please let us know so that we can cite the reference in this datasheet.

      ab247198 has not yet been referenced specifically in any publications.

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