Recombinant Anti-Legumain antibody [EPR14718] (ab183028)
![Western blot - Anti-Legumain antibody [EPR14718] (ab183028)](https://www.abcam.com/ps/products/183/ab183028/Images/ab183028-215694-anti-legumain-antibody-epr14718-western-blot.jpg "Western blot - Anti-Legumain antibody [EPR14718] (ab183028)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14718] to Legumain
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Legumain antibody [EPR14718]
See all Legumain primary antibodies -
Description
Rabbit monoclonal [EPR14718] to Legumain -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human Legumain aa 200-350. The exact sequence is proprietary.
Database link: Q99538 -
Positive control
- Human fetal kidney lysate; JAR, HeLa and 293 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14718 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab183028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 49 kDa. |
Target
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Function
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. -
Tissue specificity
Ubiquitous. Particularly abundant in kidney, heart and placenta. -
Sequence similarities
Belongs to the peptidase C13 family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 5641 Human
- Entrez Gene: 19141 Mouse
- Entrez Gene: 63865 Rat
- Omim: 602620 Human
- SwissProt: Q99538 Human
- SwissProt: O89017 Mouse
- SwissProt: Q9R0J8 Rat
- Unigene: 726036 Human
see all -
Alternative names
- AEP antibody
- Asparaginyl endopeptidase antibody
- cysteine 1 antibody
see all
Images
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Western blot - Anti-Legumain antibody [EPR14718] (ab183028)All lanes : Anti-Legumain antibody [EPR14718] (ab183028) at 1/10000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : JAR cell ysate
Lane 3 : HeLa cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 49 kDa
Exposure time: 3 minutesActual band at: 36kDa, 46kDa, 56kDa
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![Anti-Legumain antibody [EPR14718] (ab183028)](https://www.abcam.com/ps/products/183/ab183028/Images/ab183028-2-benefits-of-recombinant-antibodies.png)
Anti-Legumain antibody [EPR14718] (ab183028)
References (2)
ab183028 has been referenced in 2 publications.
- Wang C et al. Lysophosphatidic acid induces neuronal cell death via activation of asparagine endopeptidase in cerebral ischemia-reperfusion injury. Exp Neurol 306:1-9 (2018). WB ; Rat . PubMed: 29673933
- Završnik J et al. Cystatin C deficiency suppresses tumor growth in a breast cancer model through decreased proliferation of tumor cells. Oncotarget 8:73793-73809 (2017). PubMed: 29088746
