Recombinant
RabMAb

Recombinant Anti-Legumain antibody [EPR14718] (ab183028)

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Overview

  • Product name

    Anti-Legumain antibody [EPR14718]
    See all Legumain primary antibodies

  • Description

    Rabbit monoclonal [EPR14718] to Legumain

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Legumain aa 200-350. The exact sequence is proprietary.
    Database link: Q99538

  • Positive control

    • Human fetal kidney lysate; JAR, HeLa and 293 cell lysates.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 49 kDa.

Target

  • Function

    Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

  • Tissue specificity

    Ubiquitous. Particularly abundant in kidney, heart and placenta.

  • Sequence similarities

    Belongs to the peptidase C13 family.

  • Post-translational
    modifications

    Glycosylated.

  • Cellular localization

    Lysosome.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AEP antibody
    • Asparaginyl endopeptidase antibody
    • cysteine 1 antibody
    • Cysteine protease 1 antibody
    • EC 3.4.22.34 antibody
    • Legumain antibody
    • LGMN antibody
    • LGMN_HUMAN antibody
    • LGMN1 antibody
    • Protease antibody
    • Protease cysteine 1 (legumain) antibody
    • Protease cysteine 1 antibody
    • PRSC1 antibody
    see all

Images

  • Western blot - Anti-Legumain antibody [EPR14718] (ab183028)
    Western blot - Anti-Legumain antibody [EPR14718] (ab183028)
    All lanes : Anti-Legumain antibody [EPR14718] (ab183028) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : JAR cell ysate
    Lane 3 : HeLa cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 49 kDa


    Exposure time: 3 minutes


    Actual band at: 36kDa, 46kDa, 56kDa

     

References

This product has been referenced in:

  • Wang C  et al. Lysophosphatidic acid induces neuronal cell death via activation of asparagine endopeptidase in cerebral ischemia-reperfusion injury. Exp Neurol 306:1-9 (2018). WB ; Rat . Read more (PubMed: 29673933) »
  • Završnik J  et al. Cystatin C deficiency suppresses tumor growth in a breast cancer model through decreased proliferation of tumor cells. Oncotarget 8:73793-73809 (2017). Read more (PubMed: 29088746) »
See all 2 Publications for this product

Publishing research using ab183028? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-Legumain antibody [EPR14718]

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (CHO cells)
Gel Running Conditions
Reduced Denaturing (4-12%Nupage, MOPS buffer)
Loading amount
2000 cells
Specification
CHO cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

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Verified customer

Submitted Aug 07 2018

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