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RecombinantRabMAb

Recombinant Anti-Legumain antibody [EPR14718] (ab183028)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (2)

Product price, shipping and contact information

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Western blot - Anti-Legumain antibody [EPR14718] (ab183028)
  • Anti-Legumain antibody [EPR14718] (ab183028)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14718] to Legumain
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Legumain antibody [EPR14718]
    See all Legumain primary antibodies
  • Description

    Rabbit monoclonal [EPR14718] to Legumain
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Legumain aa 200-350. The exact sequence is proprietary.
    Database link: Q99538

  • Positive control

    • Human fetal kidney lysate; JAR, HeLa and 293 cell lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14718
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • Non-CD
    • APC
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Cell Biology
    • Other Antibodies
    • Plant related targets
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis

Associated products

  • Alternative Versions

    • Anti-Legumain antibody [EPR14718] - BSA and Azide free (ab232627)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Legumain protein (ab151680)
  • Related Products

    • Recombinant Human Legumain protein (ab151680)

Applications

Our Abpromise guarantee covers the use of ab183028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 49 kDa.

Target

  • Function

    Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
  • Tissue specificity

    Ubiquitous. Particularly abundant in kidney, heart and placenta.
  • Sequence similarities

    Belongs to the peptidase C13 family.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Lysosome.
  • Target information above from: UniProt accession Q99538 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5641 Human
    • Entrez Gene: 19141 Mouse
    • Entrez Gene: 63865 Rat
    • Omim: 602620 Human
    • SwissProt: Q99538 Human
    • SwissProt: O89017 Mouse
    • SwissProt: Q9R0J8 Rat
    • Unigene: 726036 Human
    • Unigene: 17185 Mouse
    • Unigene: 206021 Rat
    see all
  • Alternative names

    • AEP antibody
    • Asparaginyl endopeptidase antibody
    • cysteine 1 antibody
    • Cysteine protease 1 antibody
    • EC 3.4.22.34 antibody
    • Legumain antibody
    • LGMN antibody
    • LGMN_HUMAN antibody
    • LGMN1 antibody
    • Protease antibody
    • Protease cysteine 1 (legumain) antibody
    • Protease cysteine 1 antibody
    • PRSC1 antibody
    see all

Images

  • Western blot - Anti-Legumain antibody [EPR14718] (ab183028)
    Western blot - Anti-Legumain antibody [EPR14718] (ab183028)
    All lanes : Anti-Legumain antibody [EPR14718] (ab183028) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : JAR cell ysate
    Lane 3 : HeLa cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 49 kDa


    Exposure time: 3 minutes


    Actual band at: 36kDa, 46kDa, 56kDa

     

  • Anti-Legumain antibody [EPR14718] (ab183028)
    Anti-Legumain antibody [EPR14718] (ab183028)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (2)

    Publishing research using ab183028? Please let us know so that we can cite the reference in this datasheet.

    ab183028 has been referenced in 2 publications.

    • Wang C  et al. Lysophosphatidic acid induces neuronal cell death via activation of asparagine endopeptidase in cerebral ischemia-reperfusion injury. Exp Neurol 306:1-9 (2018). WB ; Rat . PubMed: 29673933
    • Završnik J  et al. Cystatin C deficiency suppresses tumor growth in a breast cancer model through decreased proliferation of tumor cells. Oncotarget 8:73793-73809 (2017). PubMed: 29088746

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Legumain antibody [EPR14718]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chinese hamster Cell lysate - whole cell (CHO cells)
    Gel Running Conditions
    Reduced Denaturing (4-12%Nupage, MOPS buffer)
    Loading amount
    2000 cells
    Specification
    CHO cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 07 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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