Recombinant
RabMAb

Recombinant Anti-Legumain antibody [EPR14718] - BSA and Azide free (ab232627)

Overview

  • Product name

    Anti-Legumain antibody [EPR14718] - BSA and Azide free
    See all Legumain primary antibodies
  • Description

    Rabbit monoclonal [EPR14718] to Legumain - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Legumain aa 200-350. The exact sequence is proprietary.
    Database link: Q99538

  • Positive control

    • WB: Human fetal kidney tissue lysate; JAR, HeLa and HEK-293 whole cell lysates.
  • General notes

    Ab232627 is the carrier-free version of ab183028. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232627 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.

Target

  • Function

    Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
  • Tissue specificity

    Ubiquitous. Particularly abundant in kidney, heart and placenta.
  • Sequence similarities

    Belongs to the peptidase C13 family.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Lysosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • AEP antibody
    • Asparaginyl endopeptidase antibody
    • cysteine 1 antibody
    • Cysteine protease 1 antibody
    • EC 3.4.22.34 antibody
    • Legumain antibody
    • LGMN antibody
    • LGMN_HUMAN antibody
    • LGMN1 antibody
    • Protease antibody
    • Protease cysteine 1 (legumain) antibody
    • Protease cysteine 1 antibody
    • PRSC1 antibody
    see all

Images

  • All lanes : Anti-Legumain antibody [EPR14718] (ab183028) at 1/10000 dilution

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : JAR (human placenta choriocarcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 49 kDa


    Exposure time: 3 minutes


    Actual band at: 36kDa, 46kDa, 56kDa.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183028).

References

ab232627 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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