Overview

  • Product name

    Anti-Leiomodin-3 antibody
  • Description

    Rabbit polyclonal to Leiomodin-3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    amino acids 191-280 (VTDKAFKEQR DRPEAQEQSE KKISKLDPKK LALDTSFLKV STRPSGNQTD LDGSLRRVRK NDPDMKELNL NNIENIPKEM LLDFVNAMKK) of Human Leiomodin-3.

  • Positive control

    • Human kidney tissue; Human liver lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus. Recommended concentration of ab122305 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122305, at 1/600, staining Leiomodin-3 in paraffin embedded Human kidney tissue by Immunohistochemistry.

References

ab122305 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for your patience.


I am pleased to let you know the producer of this antibody has got back to us. They have confirmed that the process of reviewing the WB data will not be completed until after the summer vacations, however they have recommended we remove WB from the tested applications list for now. I am sorry this means we are no longer able to guarantee this antibody for western blot. We appreciate that you brought this to our attention and sincerely apologize for the inconvenience. As mentioned, the data for this antibodywill be under investigation now. If thereis any further news once this is completed, I will get back to you. We will be contacting previous customers to letthem know.

They have also discussed the IHC staining pattern and expression withtheir annotation and validation specialists, regarding the expression in renal tissue. They comment that there is very little literature available for this antibody, save for the study your customer refers to which was carried our in mouse.

The IHC staining shows strong cytoplasmic positivity in small intestine, gall bladder, Purkinje cells in addition to renal tubules. Moderate staining was displayed in Bronchus, lung, hepatocytes, exocrine pancreas, myocytes and testis, where as other normal tissues were in general weakly stained or negative. They believe in the overall IHC staining of this antibody, which also includes smooth muscle cells as indicated in the article, based on the staining pattern and the information that is available at this time.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your enquiry.

I can confirm that this particular antibody is sourced externally. Regrettably, they do not have any additional WB images for this LMOD3 antibody (ab122305). Reviewing the WB image, I understand yourconcerns, as the band indeed seems to be only around 40 kDa. Our producer will need to consult withtheir Quality Control team for their opinion regarding the WB validation. Asthey are currently in the middle of summer vacations,unfortunately theymay not be able to give us any additional information until August. This is regrettably longer than we would want our customers to wait and I appreciate your patience.

This feedback is very important to us, and we will follow this up and take appropriate action if necessary once we have further information from them. I will keep you informed.
In the meantime, I would like to reassure you that as the antibody has been tested in western blot, IHC-P and human sample, and we can provide our 6 month guarantee. If the antibody does not work in the applications and species listed, then we can provide a refund, free of charge replacement or credit note.

I will get back to you as soon as we have further information. If you have any further questions, please do not hesitate to let me know.

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