Overview

  • Product name

    Anti-Lens Fiber Cell Marker antibody [ZL-1]
  • Description

    Mouse monoclonal [ZL-1] to Lens Fiber Cell Marker
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Zebrafish
    Does not react with: Human
  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab185979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance

    The lens fibers form the bulk of the lens. They are long, thin, transparent cells, firmly packed, with diameters typically between 4-7 micrometres and lengths of up to 12 mm long. These tightly packed layers of lens fibers are referred to as laminae. The lens fibers are linked together via gap junctions and interdigitations of the cells that resemble "ball and socket" forms. The lens is split into regions depending on the age of the lens fibers of a particular layer. Moving outwards from the central, oldest layer, the lens is split into an embryonic nucleus, the fetal nucleus, the adult nucleus, and the outer cortex. New lens fibers, generated from the lens epithelium, are added to the outer cortex. Mature lens fibers have no organelles or nuclei
  • Alternative names

    • Lens fiber cell Marker antibody
    • zl1 antibody

Images

  • ab185979 staining the lens in 4 dpf zebrafish embryo retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and permeabilization was performed using 0.5% Triton X. Blocking of the sample was done with 5% BSA in PBS containing 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab185979 at 1/100 in blocking solution for 16h at 4°C. An alexa 647 conjugated goat anti-mouse polyclonal antibody at 1/500 was used as the secondary antibody. Nuclei are stained in blue with DAPI. The lens can be observed.

    See Abreview

References

ab185979 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Lens)
Specification
Lens
Permeabilization
Yes - triton X
Fixative
Paraformaldehyde

Dr. Ryan Macdonald

Verified customer

Submitted May 01 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up