Key features and details
- Rabbit polyclonal to LEPRE1/P3H1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LEPRE1/P3H1 antibody
See all LEPRE1/P3H1 primary antibodies
DescriptionRabbit polyclonal to LEPRE1/P3H1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human LEPRE1/P3H1 aa 200-250. The exact sequence is proprietary.
Database link: Q32P28
- WB: HeLa, HEK-293T and Jurkat whole cell lysates.
This product was previously labelled as LEPRE1
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241480 was affinity purified using an epitope specific to LEPRE1/P3H1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 83 kDa.|
FunctionBasement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts.
Involvement in diseaseDefects in LEPRE1 are the cause of osteogenesis imperfecta type 8 (OI8) [MIM:610915]. A connective tissue disorder characterized by disproportionate short stature, severe osteoporosis, shortening of the long bones, white sclerae, a round face and a short barrel-shaped chest.
Sequence similaritiesBelongs to the leprecan family.
Contains 1 Fe2OG dioxygenase domain.
Contains 4 TPR repeats.
modificationsO-glycosylated; chondroitin sulfate.
Cellular localizationEndoplasmic reticulum. Secreted > extracellular space > extracellular matrix. Secreted into the extracellular matrix as a chondroitin sulfate proteoglycan.
- Information by UniProt
- GROS 1 antibody
- GROS1 antibody
- Growth suppressor 1 antibody
All lanes : Anti-LEPRE1/P3H1 antibody (ab241480) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 83 kDa
Exposure time: 3 minutes
Lysates prepared in NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241480 has not yet been referenced specifically in any publications.