Recombinant
RabMAb

Recombinant Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (Alexa Fluor® 488) (ab223430)

Overview

  • Product name

    Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (Alexa Fluor® 488)
    See all LEPRE1/P3H1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10193(B)] to LEPRE1/P3H1 (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human LEPRE1/P3H1 aa 700 to the C-terminus.
    Database link: Q32P28

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt: HeLa cells
  • General notes

     This product was previously labelled as LEPRE1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab223430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

Flow Cyt 1/500.

Target

  • Function

    Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts.
  • Involvement in disease

    Defects in LEPRE1 are the cause of osteogenesis imperfecta type 8 (OI8) [MIM:610915]. A connective tissue disorder characterized by disproportionate short stature, severe osteoporosis, shortening of the long bones, white sclerae, a round face and a short barrel-shaped chest.
  • Sequence similarities

    Belongs to the leprecan family.
    Contains 1 Fe2OG dioxygenase domain.
    Contains 4 TPR repeats.
  • Post-translational
    modifications

    O-glycosylated; chondroitin sulfate.
  • Cellular localization

    Endoplasmic reticulum. Secreted > extracellular space > extracellular matrix. Secreted into the extracellular matrix as a chondroitin sulfate proteoglycan.
  • Information by UniProt
  • Database links

  • Alternative names

    • GROS 1 antibody
    • GROS1 antibody
    • Growth suppressor 1 antibody
    • LEPRE 1 antibody
    • Lepre1 antibody
    • Leprecan 1 antibody
    • Leprecan antibody
    • Leprecan-1 antibody
    • Leprecan1 antibody
    • Leucine proline enriched proteoglycan (leprecan) 1 antibody
    • Leucine proline enriched proteoglycan 1 antibody
    • Leucine- and proline-enriched proteoglycan 1 antibody
    • MGC117314 antibody
    • OI8 antibody
    • P3H1 antibody
    • P3H1_HUMAN antibody
    • Prolyl 3 hydroxylase 1 antibody
    • Prolyl 3-hydroxylase 1 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab223430 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223430, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • ab223430 staining LEPRE1/P3H1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223430 at 1/1000 dilution (shown in Green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab223430 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223430.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up