- Datasheet
- Specific References (11)
- Protocols
Overview
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Product nameAnti-Leptin antibody
See all Leptin primary antibodies -
DescriptionRabbit polyclonal to Leptin
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IP, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Sheep, Rabbit, Human, Pig
Predicted to work with: Goat, Horse, Cow, Cat, Dog, Chimpanzee, Macaque monkey, Gorilla
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Immunogen
Synthetic peptide:
SRNVIQISNDLENLRD
, corresponding to amino acids 91-106 of Human Leptin. -
Positive control
- Human Serum
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityAmmonium Sulphate Precipitation
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab16227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/20 - 1/200. | |
| IP | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/5000. Detects a band of approximately 16 kDa. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
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Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
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Sequence similaritiesBelongs to the leptin family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 493838 Cat
- Entrez Gene: 449638 Chimpanzee
- Entrez Gene: 280836 Cow
- Entrez Gene: 3952 Human
- Entrez Gene: 16846 Mouse
- Entrez Gene: 396832 Pig
- Omim: 164160 Human
- SwissProt: Q9N2C1 Cat
see all -
Alternative names
- FLJ94114 antibody
- LEP antibody
- LEP_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab16227)Immunocytochemistry/Immunofluorescent analysis of Leptin (green) showing staining in the secretion of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab16227 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leptin antibody (ab16227)Ab16227 staining human placenta.
Left panel: with primary antibody. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Western blot - Anti-Leptin antibody (ab16227) -
Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab16227)Immunocytochemistry/Immunofluorescent analysis of Leptin (green) showing staining in the secretion of HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab16227 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
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Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab16227)Immunocytochemistry/Immunofluorescent analysis of Leptin (green) showing staining in the secretion of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab16227 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
Protocols
References
This product has been referenced in:
- Sun C et al. Leptin-induced inflammation by activating IL-6 expression contributes to the fibrosis and hypertrophy of ligamentum flavum in lumbar spinal canal stenosis. Biosci Rep 38:N/A (2018). Read more (PubMed: 29436483) »
- Pan Y et al. Leptin-LepRb Expressed in Gastric Cancer Patients and Related to Cancer-Related Depression. Biomed Res Int 2017:6482842 (2017). Read more (PubMed: 28316984) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"