Anti-Leptin antibody (ab16227)

Thank you for your reply.

The lab confirmed that leptin is cytoplasmic and not nuclear. The image as well as the legend shown on the product datasheet are therefore incorrect and I asked my colleagues responsible for the website to remove or update the information. Thank you for highlighting this mistake to us.

Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

As explained in my previous email, when a product is to be used in an untested species or application, end users may be eligible for our AbTrial program.

Please reply to this email if you are interested in testing this product and I will send you an AbTrial discount code. After submitting your results the discount code will become active and will be useable on your next order to receive that value off. The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

Please note that This item is currently out of stock and is estimated to be available by late-November 2012. We apologise for any inconvenience. You may prefer to test another anti-Leptin instead. The link to the list of all Abcam anti-Leptin antibodies is : https://www.abcam.com/index.html?t=58&pt=1.

The last lots of ab3583 we had in stock had a concentration of 0.7 mg/ml, the unit size is indeed 50µl.

I hope this is helpful to you. Do not hesitate to contact me again if you have questions.

Thank you very much for your interest in our anti-Leptin ab16227.

The IHC-P image comes from an end-user's feedback, not from the characterisation lab.

We always welcome valuable feedback from our customers, so if you would like to provide your own feedback on this product in IHC-P and with Dog samples, you can participate in our AbTrial program and use our products without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.
2. Our scientists will email you an inactive personal discount code for the value of the product.
3. Purchase and test the product at the regular price.
4. Submit your results, including your discount code in the additional notes section of your Abreview.
5. Once the Abreview is submitted, the discount code will become active.
6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

Thank you for your enquiry.

I can confirm that ab3583 and ab16227 antibodiesshould detect leptin both intra and extracellularlly.However based on the references for these product it appears that most if not all of the leptin is immediately secreted and would therefore be found extracellularlly.

I am sorry that as far as we are aware, these antibodies have not specifically been tested inchondrocyts.However, I would like to reassure you that our antibodies are tested and covered by our 6 month guarantee in the listed tested applications and species on the individual datasheets.

I hope this information is helpful. If you have any further questions, please do not hesitate to contact us.

The antibody ab16227 is not conjugated to anything that will produce color. To use this for ELISA, you will need an anti-rabbit IgG antibody, conjugated to an enzyme that will catalyze a color-producing reaction. This enzyme is typically horseradish peroxidase, or HRP for short. One example of such a secondary antibody is ab97064.

However, I am concerned that your meaning for "secondary" is different from mine. Are you trying to do a sandwich ELISA? If so you will need a capture antibody, coated onto the plate, and a detection antibody, which sticks to the leptin that has been captured. If this is what you want to do, the best pair of antibodies for you, assuming your samples are of human origin, will probably be a rabbit polyclonal antibody raised agains the full protein, such as ab13986, and ab6055 for detection, which is directly conjugated to HRP and does not need a secondary anti-IgG.

You will still need a substrate and chromagen for development of color, which we do not have in our catalogue yet.

Please let us know if you have any questions.

Thank you for your enquiry. I have just quickly checked the datahseets and the specificity of these products for you and can confirm that these slides can be used as positive control. - ab4616 for ab62551 and - ab4360 for ab16227 I would suggest following the staining procedure (provided in the legend og the IHC image) and perhaps applying signal enhancing step (Dako Envision Flex amplification or ABC system). I hope this helps and if I can assist further, please do not hesitate to contact me.

Thank you for your enquiry regarding ab16227 and your interest in our products. This antibody to Leptin has not been tested in Bovine. The sequence between human and bovine leptin only differ by 1 a.a. residue, so I would expect it to work, but we have not tested this product in Bovine. I hope this information helps.

Thank you for your enquiry. First, I would like to apologize if I spelled your name incorrectly. It appears that some of the characters in your name did not email properly. It appears that ab16227 is the only leptin antibody we carry that is predicted to react with cow tissue, and as you noted, this antibody has not been tested in ELISA. It is possible that the antibody would work, but we have not tested it. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abpoints which can be redeemed on a number of rewards (a further 100 Abpoints will be offered for an image). I hope this information helps. Please do not hesitate to contact us if you need anything further.