Overview

  • Product name
  • Description
    Chicken polyclonal to Leptin
  • Host species
    Chicken
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein (Rat)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab28955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/5000.
WB 1/1000 - 1/5000. Detects a band of approximately 16 kDa.

Target

  • Function
    May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in disease
    Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similarities
    Belongs to the leptin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ94114 antibody
    • LEP antibody
    • LEP_HUMAN antibody
    • LEPD antibody
    • Leptin (murine obesity homolog) antibody
    • Leptin (obesity homolog, mouse) antibody
    • Leptin antibody
    • Leptin Murine Obesity Homolog antibody
    • Leptin Precursor Obesity Factor antibody
    • OB antibody
    • Obese protein antibody
    • Obese, mouse, homolog of antibody
    • Obesity antibody
    • Obesity factor antibody
    • Obesity homolog mouse antibody
    • Obesity Murine Homolog Leptin antibody
    • OBS antibody
    • OTTHUMP00000212285 antibody
    see all

References

ab28955 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for letting me know.

I am sorry ab28955 has not been working for you in western blot. I am sending you a positive control leptin protein free of charge. ab73532 has a 2-3 week wait time, so I sent you ab646 (https://www.abcam.com/leptin-protein-ab646.html), which is expected to be delivered ****

Your order number for ab646 is Order ****

Please let me know if the antibody properly detects the leptin protein I am sending you. If it does not, I will be happy to refund or replace ab28955 free of charge.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for your reply.

I appreciate your sharing of the protocol with me. The only reason I think it may be worth trying the 20% gel is that your lower percentage gels may cause the protein to run off your gel since the 16 kDa portion of your ladder is near the end of your gel and the leptin protein can at times show up at 14-15 kDa . Thus, I would try running with the 20% gel, taking care that the 16kDa mark on the ladder does not get close to the end of the gel.

If this does not work, I would like to send you a positive control rat leptin protein (ab73532) free of charge to determine if the antibody is not properly detecting leptin or if your samples simply have levels of leptin that are too low to detect.

Lastly, if the rat leptin protein ab73532 does not yield a band around 16 kDa, I would be happy to send you a free of charge replacement (such as ab7210 that you mentioned).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for contacting Abcam.

I am sorry to hear that you have been experiencing issues when using this antibody. This product is covered by our Abpromise guarantee. According to Abpromise guarantee, if this product has been purchased within the last six months, we are happy to provide scientific support and to replace or refund the product should we not be able to help.

Would you be able to provide more details regarding your protocol and troubleshooting step that have been taken? These will not only help me to fully understand the problem but are essential should further testing of this product be needed as well.
Specifically:
1. What blocking solution did you use?
2. Did you reduce and denature your samples? We recommend boiling in SDS + beta-mercaptoethanol or DTT for 5 minutes at 95oC prior to loading.
3. What was the percentage of acrylamide in gel? For proteins of 4-40 kDa we recommend 20% acrylamide gel
4. Did you run positive and negative controls?

I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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