Overview

  • Product name
  • Description
    Rabbit polyclonal to Leptin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab7208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 µg/ml.

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab20101 as Capture.

WB 1/2000.

Target

  • Function
    May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in disease
    Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similarities
    Belongs to the leptin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ94114 antibody
    • LEP antibody
    • LEP_HUMAN antibody
    • LEPD antibody
    • Leptin (murine obesity homolog) antibody
    • Leptin (obesity homolog, mouse) antibody
    • Leptin antibody
    • Leptin Murine Obesity Homolog antibody
    • Leptin Precursor Obesity Factor antibody
    • OB antibody
    • Obese protein antibody
    • Obese, mouse, homolog of antibody
    • Obesity antibody
    • Obesity factor antibody
    • Obesity homolog mouse antibody
    • Obesity Murine Homolog Leptin antibody
    • OBS antibody
    • OTTHUMP00000212285 antibody
    see all

Images

References

This product has been referenced in:
  • Xie X  et al. Characterization of the Human Adipocyte Proteome and Reproducibility of Protein Abundance by One-Dimensional Gel Electrophoresis and HPLC-ESI-MS/MS. J Proteome Res 9:4521-34 (2010). Read more (PubMed: 20812759) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

The HRP conjugated anti-Leptin ab6055 has been tested in sELISA with ab6057 (https://www.abcam.com/ab6057). We do not have any data for the pair ab6055 / ab7208.

I would not recommend to use a secondary to detect a conjugated antibody.

Here is the list of the two alternative antibody pairs used in sELISA for the detection of Human Leptin that may be suitable for your work:

ab20101 / ab7208. Both antibodies are unconjugated: https://www.abcam.com/ab20101 and https://www.abcam.com/ab7208.
ab6056 / ab36498. Both antibodies are unconjugated: https://www.abcam.com/ab6056 and https://www.abcam.com/ab36498.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us and your interest in our products.

We have a number of different antibodies directed against the human Leptin protein. The ideal choices for your experiment would depend on what application you are hoping to use.

We have several antibodies which are able to detect different epitopes from the Leptinprotein such as ab50424 which is a goat polyclonal raised againsta synthetic peptide derived from the residues 829-841 of human Leptin. This antibody has been used in ELISA and western blotting.

The antibodyab20101 (mouse monoclonal)has been raised against the peptide taken from residues 131-145 of human Leptin. This antibody has been used in sandwich ELISA, Western blotting and IHC. The antibody ab20101 was used as an ELISA sandwich pair with the rabbit polyclonal ab7208 which was raised against the full length human Leptin protein. The standard curve was performed using the recombinantly expressed human Leptinprotein (ab51273).

In order to advise which secondary antibody (anti-IgG of host animal) would be suitable, I would need to know which conjugate you would like to use in your assay. It would also be helpful to know the application in which you wish to use the antibodies. If you could provide me with this information I will be able to advise you further. If you could let me knowwhich antibodies you'd be interested in I can also let you know the delivery time and how much antibody would be provided (alternatively this information isavailable on the datasheet of each product).

All of our products are for researchpurposes only and are not intended for diagnostic, therapeutic or in vivo use.

I hope this information has been of some help. If you require any further information please do not hesitate to contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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