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I would like to set up a sandwich immunoassay for leptin, employing either HRP or fluorescent nanospheres (prepared in my lab) for detection.
Do you think I could couple the AB6055 raised in mouse (to be used as a detection antibody) with the AB7208 raised in rabbit (to be used as a capture antibody)?
If so, do you think I could also employ my nanosphere-labeled anti-mouse secondary antibody to bind to AB6055 instead of using HRP for detection?
Asked on Oct 10 2012
Thank you for contacting us.
The HRP conjugated anti-Leptin ab6055 has been tested in sELISA with ab6057 (https://www.abcam.com/ab6057). We do not have any data for the pair ab6055 / ab7208.
I would not recommend to use a secondary to detect a conjugated antibody.
Here is the list of the two alternative antibody pairs used in sELISA for the detection of Human Leptin that may be suitable for your work:
ab20101 / ab7208. Both antibodies are unconjugated: https://www.abcam.com/ab20101 and https://www.abcam.com/ab7208.
ab6056 / ab36498. Both antibodies are unconjugated: https://www.abcam.com/ab6056 and https://www.abcam.com/ab36498.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Oct 10 2012