Anti-Leptin antibody (ab9826)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Leptin antibody
See all Leptin primary antibodies -
Description
Rabbit polyclonal to Leptin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Highly pure (>98%) recombinant human Leptin
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Positive control
- Purchase matching WB positive control:Recombinant Human Leptin protein
- Recombinant Human Leptin protein (ab9827) can be used as a positive control in WB.
Properties
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Form
Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
PBS, pH 7.4, no preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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ELISA pair antibody
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab9826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.2 µg/ml. | |
| ELISA | Use a concentration of 0.5 µg/ml. Can be paired for ELISA with Mouse monoclonal to Leptin (ab9828). Allows the detection of 0.2 - 0.4 ng/well of recombinant human Leptin (using 100µl/well antibody solution). |
Target
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Function
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
Involvement in disease
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
Sequence similarities
Belongs to the leptin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3952 Human
- Omim: 164160 Human
- SwissProt: P41159 Human
- Unigene: 194236 Human
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Alternative names
- FLJ94114 antibody
- LEP antibody
- LEP_HUMAN antibody
see all
Protocols
Datasheets and documents
References
ab9826 has not yet been referenced specifically in any publications.
