Anti-Leptin antibody (ab9826)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Leptin antibody
See all Leptin primary antibodies -
DescriptionRabbit polyclonal to Leptin
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Human
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Immunogen
Highly pure (>98%) recombinant human Leptin
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Positive control
- Purchase matching WB positive control:Recombinant Human Leptin protein
- Recombinant Human Leptin protein (ab9827) can be used as a positive control in WB.
Properties
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FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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Isotypeunknown
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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ELISA pair antibody
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab9826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.2 µg/ml. | |
| ELISA | Use a concentration of 0.5 µg/ml. Can be paired for ELISA with Mouse monoclonal to Leptin (ab9828). Allows the detection of 0.2 - 0.4 ng/well of recombinant human Leptin (using 100µl/well antibody solution). |
Target
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FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
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Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
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Sequence similaritiesBelongs to the leptin family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3952 Human
- Omim: 164160 Human
- SwissProt: P41159 Human
- Unigene: 194236 Human
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Alternative names
- FLJ94114 antibody
- LEP antibody
- LEP_HUMAN antibody
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Protocols
Datasheets and documents
References
ab9826 has not yet been referenced specifically in any publications.
