Product nameAnti-Leptin antibody (Biotin)
See all Leptin primary antibodies
DescriptionRabbit polyclonal to Leptin (Biotin)
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Leptin aa 22-167. Mature chain. E. coli derived.
MVPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPIL TLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLP WASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC
Database link: P41159
- WB: Recombinant human Leptin.
FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 19 kDa.
Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|ELISA||Use a concentration of 0.25 - 1 µg/ml.
Human Leptin was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271278. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hLeptin protein.
FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Sequence similaritiesBelongs to the leptin family.
- Information by UniProt
- FLJ94114 antibody
- LEP antibody
- LEP_HUMAN antibody
Lane 1: Marker.
Lane 2: Recombinant hLeptin at 250ng/lane.
Lane 3: Recombinant hLeptin at 125ng/lane.
Lane 4: Recombinant hLeptin at 62.5ng/lane.
Lane 5: Recombinant hLeptin at 31.25ng/lane.
Lane 6: Recombinant hLeptin at 15.625ng/lane.
Lane 7: Recombinant hLeptin at 7.8ng/lane.
Lane 8: Recombinant hLeptin at 3.9ng/lane.
Lane 9: Recombinant hLeptin at 1.95ng/lane.
Lane 10: Recombinant hLeptin at 0.975ng/lane.
Lane 11: Recombinant hLeptin at 0.4875ng/lane.
Lane 12: Recombinant hLeptin at 0.24ng/lane.
Human Leptin was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271278. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLeptin protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271278 has not yet been referenced specifically in any publications.