The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 0.5 µg/ml.
Note: detects 0.2 - 0.4 ng/well of recombinant leptin.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 19 kDa.
Notes: detects 1.5 - 3.0 ng/lane of recombinant leptin under either reducing or non-reducing conditions
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Involvement in disease
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.