Key features and details
- Goat polyclonal to Leptin (Biotin)
- Suitable for: Sandwich ELISA, WB
- Reacts with: Rat
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-Leptin antibody (Biotin)
See all Leptin primary antibodies
DescriptionGoat polyclonal to Leptin (Biotin)
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Rat
Highly pure (>98%) recombinant Rat Leptin
FormLyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab84017 is sterile filtered.
Our Abpromise guarantee covers the use of ab84017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
Allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat Leptin.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 19 kDa.
The detection limit for recombinant Rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Sequence similaritiesBelongs to the leptin family.
- Information by UniProt
- FLJ94114 antibody
- LEP antibody
- LEP_HUMAN antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab84017 has not yet been referenced specifically in any publications.