Overview

  • Product name
    Anti-Leptin antibody [EPR16848-34]
    See all Leptin primary antibodies
  • Description
    Rabbit monoclonal [EPR16848-34] to Leptin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Blocking, Functional Studiesmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein within Mouse Leptin aa 1-200. The exact sequence is proprietary.
    Database link: P41160

  • Positive control
    • Blocking Assay: HEK-293 cells co-transfected with a plasmid encoding the full-length mouse leptin receptor, pRL-CMV and pGL4.47[luc2P/SIE/Hygro] Vectors.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration.

(50 μg/ml-100 μg/ml).

Functional Studies Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
    • Involvement in disease
      Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
    • Sequence similarities
      Belongs to the leptin family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ94114 antibody
      • LEP antibody
      • LEP_HUMAN antibody
      • LEPD antibody
      • Leptin (murine obesity homolog) antibody
      • Leptin (obesity homolog, mouse) antibody
      • Leptin antibody
      • Leptin Murine Obesity Homolog antibody
      • Leptin Precursor Obesity Factor antibody
      • OB antibody
      • Obese protein antibody
      • Obese, mouse, homolog of antibody
      • Obesity antibody
      • Obesity factor antibody
      • Obesity homolog mouse antibody
      • Obesity Murine Homolog Leptin antibody
      • OBS antibody
      • OTTHUMP00000212285 antibody
      see all

    Images

    • HEK-293 cells were co-transfected with a plasmid encoding the full-length mouse leptin receptor, pRL-CMV and pGL4.47[luc2P/SIE/Hygro] Vectors. Dual-Luciferase® Reporter Assay was used to test the leptin signaling.

      ab181298 (0, 50, 100 µg/ml) was incubated with recombinant mouse leptin in a total of 500ul culture medium at the final concentration of 100, 50, 25, and 12.5 ng/ml for 2h at 37℃ before adding to transfected cells.

      Leptin without incubating with ab181298 as the positive control (green) shows the maximal signal of the leptin and leptin receptor interaction, whereas the base line signal (without adding leptin and ab181298 to the system) serves as the negative control (purple).  

      The data demonstrates that the interaction of leptin and leptin receptor can be blocked by ab181298 resulting in decreased florescence signals.

      The data was provided by our collaborator Dr. Bing Sun, Chinese Academy of Sciences.

    • HEK-293 cells were co-transfected with a plasmid encoding full length human leptin receptor, pRL-CMV and pGL4.47[luc2P/SIE/Hygro] Vectors.  Dual-Luciferase® Reporter Assay was used to test the leptin signaling.

      ab181298 (0, 50, 100 µg/ml) was incubated with recombinant mouse leptin in a total of 500ul culture medium at the final concentration of 100, 50, 25, and 12.5 ng/ml for 2h at 37℃ before adding to transfected cells.

      Leptin without incubating with ab181298 as the positive control (green) shows the maximal signal of the leptin and leptin receptor interaction, whereas the base line signal (without adding leptin and ab181298 to the system) serves as the negative control (purple).  

      The data demonstrates that the interaction of leptin and leptin receptor can be blocked by ab181298 resulting in decreased florescence signals.

      The data was provided by our collaborator Dr. Bing Sun, Chinese Academy of Sciences.

       

    References

    ab181298 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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