Recombinant Anti-Leptin antibody [EPR16848-34] - BSA and Azide free (ab250477)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16848-34] to Leptin - BSA and Azide free
- Suitable for: Blocking
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Leptin antibody [EPR16848-34] - BSA and Azide free
See all Leptin primary antibodies -
Description
Rabbit monoclonal [EPR16848-34] to Leptin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Blockingmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250477 is the carrier-free version of ab181298.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16848-34 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Blocking |
Use at an assay dependent concentration.
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Notes |
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Blocking
Use at an assay dependent concentration. |
Target
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Function
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
Involvement in disease
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
Sequence similarities
Belongs to the leptin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3952 Human
- Entrez Gene: 16846 Mouse
- Omim: 164160 Human
- SwissProt: P41159 Human
- SwissProt: P41160 Mouse
- Unigene: 194236 Human
- Unigene: 277072 Mouse
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Alternative names
- FLJ94114 antibody
- LEP antibody
- LEP_HUMAN antibody
see all
Images
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This data was developed using ab181298, the same antibody clone in a different buffer formulation.HEK-293 cells were co-transfected with a plasmid encoding the full-length mouse leptin receptor, pRL-CMV and pGL4.47[luc2P/SIE/Hygro] Vectors. Dual-Luciferase® Reporter Assay was used to test the leptin signaling.ab181298 (0, 50, 100 µg/ml) was incubated with recombinant mouse leptin in a total of 500ul culture medium at the final concentration of 100, 50, 25, and 12.5 ng/ml for 2h at 37°C before adding to transfected cells.Leptin without incubating with ab181298 as the positive control (green) shows the maximal signal of the leptin and leptin receptor interaction, whereas the base line signal (without adding leptin and ab181298 to the system) serves as the negative control (purple).The data demonstrates that the interaction of leptin and leptin receptor can be blocked by ab181298 resulting in decreased florescence signals.
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This data was developed using ab181298, the same antibody clone in a different buffer formulation.HEK-293 cells were co-transfected with a plasmid encoding full length human leptin receptor, pRL-CMV and pGL4.47[luc2P/SIE/Hygro] Vectors. Dual-Luciferase® Reporter Assay was used to test the leptin signaling. ab181298 (0, 50, 100 µg/ml) was incubated with recombinant mouse leptin in a total of 500ul culture medium at the final concentration of 100, 50, 25, and 12.5 ng/ml for 2h at 37? before adding to transfected cells. Leptin without incubating with ab181298 as the positive control (green) shows the maximal signal of the leptin and leptin receptor interaction, whereas the base line signal (without adding leptin and ab181298 to the system) serves as the negative control (purple). The data demonstrates that the interaction of leptin and leptin receptor can be blocked by ab181298 resulting in decreased florescence signals. The data was provided by our collaborator Dr. Bing Sun, Chinese Academy of Sciences.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250477 has not yet been referenced specifically in any publications.