Overview

  • Product name

    Anti-Leptin Receptor antibody
    See all Leptin Receptor primary antibodies
  • Description

    Rabbit polyclonal to Leptin Receptor
  • Host species

    Rabbit
  • Specificity

    Recombinant and natural mouse OB receptor.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to amino acids 217-376 of Mouse Leptin Receptor. Expressed in E. coli

Properties

Applications

Our Abpromise guarantee covers the use of ab7212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/2000. Predicted molecular weight: 120-140 kDa. The native leptin receptor is a homodimer held together with disulfide bonds. On a reducing SDS-PAGE, bands may appear as approximately 60 kDa subunits. Further variations in size is possible and is due to glycosylation.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.
    • Tissue specificity

      Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.
    • Sequence similarities

      Belongs to the type I cytokine receptor family. Type 2 subfamily.
      Contains 4 fibronectin type-III domains.
      Contains 1 Ig-like (immunoglobulin-like) domain.
    • Domain

      The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.
      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.
    • Post-translational
      modifications

      On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD 295 antibody
      • CD295 antibody
      • CD295 antigen antibody
      • Db antibody
      • Fa antibody
      • HuB219 antibody
      • LEP R antibody
      • LEP-R antibody
      • LEPR antibody
      • LEPR_HUMAN antibody
      • LEPRD antibody
      • Leptin receptor antibody
      • Leptin receptor fatty antibody
      • Leptin receptor gene related protein antibody
      • Leptin receptor precursor antibody
      • Leptin receptor precursor antibody
      • OB R gene related protein antibody
      • OB receptor antibody
      • OB-R antibody
      • OB-RGRP antibody
      • obl antibody
      • Obr antibody
      see all

    References

    This product has been referenced in:

    • Dong ZW & Yuan YF Juglanin suppresses fibrosis and inflammation response caused by LPS in acute lung injury. Int J Mol Med 41:3353-3365 (2018). Read more (PubMed: 29532887) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Question
    Answer

    This antibody has not been tested for use in immunocytochemistry.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up