Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
- Reacts with: Human
Product nameLeptospira IgM ELISA kit
See all Leptospira kits
Intra-assay Sample n Mean SD CV% sample 24 0.531nM 5.34% sample 24 1.07nM 3.32% sample 24 1.9nM 2.8% Inter-assay Sample n Mean SD CV% sample 12 20.74nM 4.35% sample 12 37.86nM 4.97% sample 12 6.79nM 8.1%
Sample typeSerum, Hep Plasma, Cit plasma
Assay typeSandwich (qualitative)
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Leptospira IgM ELISA kit (ab247200) is intended for the qualitative determination of IgM class antibodies against Leptospira spp. in human serum or plasma (heparin).
The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.
Leptospirosis (also known as Weil's syndrome) is probably the most widespread zoonosis in the world. It is caused by infection with spirochete bacteria of the genus Leptospira and affects humans as well as a broad spectrum of animal hosts. The incidence is significantly higher in warm climate countries than in temperate regions. The disease is seasonal, with peak incidence occurring in summer or fall in temperate regions, where temperature is the limiting factor in survival of leptospires, and during rainy seasons in warm climate regions, where rapid desiccation would otherwise prevent survival. Natural reservoirs for the pathogenic Leptospira interrogans include rodents as well as a large variety of domesticated mammals (e. g. pigs, cattle and dogs). Leptospires occupy the lumen of nephritic tubules in their natural host and are shed into the urine.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml anti-human IgM HRP conjugate 1 x 20ml Cover Foil 1 unit IgM Cut-off Control 1 x 3ml IgM Negative Control 1 x 2ml IgM Positive Control 1 x 2ml IgM Sample Diluent 1 x 100ml Leptospira Coated Microplate (IgM) 1 unit Stop Solution red cap 1 x 15ml TMB Substrate Solution Yellow cap 1 x 15ml
Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
ab247200 has not yet been referenced specifically in any publications.