Product nameAnti-LETM1 antibody
See all LETM1 primary antibodies
DescriptionRabbit polyclonal to LETM1
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- WB: HeLa, MCF7, HEK-293, HepG2 and Jurkat whole cell lysates; mouse heart lysate. IHC-P: Human liver cancer tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- Mouse heart normal tissue lysate - total protein (ab30291)
- MCF7 whole cell lysate (ab3871)
- Jurkat whole cell lysate (ab7899)
- HepG2 whole cell lysate (ab7900)
- HEK293 whole cell lysate (ab7902)
Our Abpromise guarantee covers the use of ab236743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 83 kDa.|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/200 - 1/500.|
FunctionCrucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain. Required for the maintenance of the tubular shape and cristae organization.
Sequence similaritiesContains 1 EF-hand domain.
Contains 1 LETM1 domain.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- LETM 1 antibody
- LETM1 and EF hand domain containing protein 1, mitochondrial antibody
- LETM1 and EF-hand domain-containing protein 1 antibody
All lanes : Anti-LETM1 antibody (ab236743) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : Mouse heart lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 83 kDa
Paraffin-embedded human liver cancer tissue stained for LETM1 using ab236743 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for LETM1 (green) using ab236743 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L). The nuclear counterstain is DAPI (blue).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.
ab236743 has not yet been referenced specifically in any publications.