Key features and details
- Rabbit polyclonal to LETM1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-LETM1 antibody
See all LETM1 primary antibodies
DescriptionRabbit polyclonal to LETM1
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- WB: HeLa, MCF7, HEK-293, HepG2 and Jurkat whole cell lysates; mouse heart lysate. IHC-P: Human liver cancer tissue. ICC/IF: HeLa cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- Mouse heart normal tissue lysate - total protein (ab30291)
- MCF7 whole cell lysate (ab3871)
- Jurkat whole cell lysate (ab7899)
- HEK293 whole cell lysate (ab7902)
Our Abpromise guarantee covers the use of ab236743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 83 kDa.|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/200 - 1/500.|
FunctionCrucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain. Required for the maintenance of the tubular shape and cristae organization.
Sequence similaritiesContains 1 EF-hand domain.
Contains 1 LETM1 domain.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- LETM 1 antibody
- LETM1 and EF hand domain containing protein 1, mitochondrial antibody
- LETM1 and EF-hand domain-containing protein 1 antibody
All lanes : Anti-LETM1 antibody (ab236743) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : Mouse heart lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 83 kDa
Paraffin-embedded human liver cancer tissue stained for LETM1 using ab236743 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for LETM1 (green) using ab236743 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L). The nuclear counterstain is DAPI (blue).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236743 has not yet been referenced specifically in any publications.