Product nameAnti-LETMD1/HCCR-1 antibody
See all LETMD1/HCCR-1 primary antibodies
DescriptionRabbit polyclonal to LETMD1/HCCR-1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Recombinant full length protein corresponding to Human LETMD1/HCCR-1 aa 1-360.
MALSRVCWARSAVWGSAVTPGHFVTRRLQLGRSGLAWGAPRSSKLHLSPK ADVKNLMSYVVTKTKAINGKYHRFLGRHFPRFYVLYTIFMKGLQMLWADA KKARRIKTNMWKHNIKFHQLPYREMEHLRQFRQDVTKCLFLGIISIPPFA NYLVFLLMYLFPRQLLIRHFWTPKQQTDFLDIYHAFRKQSHPEIISYLEK VIPLISDAGLRWRLTDLCTKIQRGTHPAIHDILALRECFSNHPLGMNQLQ ALHVKALSRAMLLTSYLPPPLLRHRLKTHTTVIHQLDKALAKLGIGQLTA QEVKSACYLRGLNSTHIGEDRCRTWLGEWLQISCSLKEAELSLLLHNVVL LSTNYLGTRR
Database link: Q6P1Q0
- PANC1 cell extract.
This product was previously labelled as LETMD1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab175410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/50 - 1/200.|
RelevanceLETMD1 is involved in tumorigenesis and may function as a negative regulator of the TP53/p53. It is found in kidney, liver, skeletal muscle, heart and brain and is over-expressed in various tumors including leukemia, lymphoma, and carcinomas of the breast, kidney, ovary, stomach, colon and uterine cervix. There are six isoforms.
Cellular localizationMembrane; Single-pass membrane protein
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Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab175410. Blue DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded rat kidney damage using ab175410 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver damage using ab175410 at dilution of 1:100
All lanes : Anti-LETMD1/HCCR-1 antibody (ab175410) at 1/1000 dilution
Lane 1 : MCF7 cell lysate with 3% nonfat dry milk in TBST.
Lane 2 : Mouse liver lysate with 3% nonfat dry milk in TBST.
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 42 kDa
Exposure time: 90 seconds
Anti-LETMD1/HCCR-1 antibody (ab175410) at 1/500 dilution + PANC1 cell extract
Predicted band size: 42 kDa
ab175410 has not yet been referenced specifically in any publications.