Overview

  • Product name

    Anti-Leu Enkephalin antibody [NOC1]
    See all Leu Enkephalin primary antibodies
  • Description

    Mouse monoclonal [NOC1] to Leu Enkephalin
  • Host species

    Mouse
  • Specificity

    This antibody does not distinguish between Met-enkephalin and Leu-enkephalin by radioimmunoassay or by immunocytochemistry.

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, RIA, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Leu Enkephalin conjugated to bovine serum albumin.
    Sequence:

    Tyr-Gly-Gly-Phe-Leu

Properties

Applications

Our Abpromise guarantee covers the use of ab150346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
IHC-Fr Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
ICC 1/200.

Target

  • Relevance

    Enkephalin and enkephalin-like peptides have been detected in many animal species. In mammals these opioid pentapeptides are cleaved from a common precursor molecule which is expressed in a number of cell types, including neurons and endocrine cells associated with the sympathetic nervous system where they are often co-stored with catecholamines. They are known to be endogenous ligands for Sopioid receptors and are known to mediate analgesia at the spinal level.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • PENK antibody
    • Proenkephalin A antibody
    • Proenkephalin antibody

Images

  • Paraformaldehyde-fixed, paraffin-embedded mouse brain tissue stained for Leu Enkephalin using ab150346 at 1/100 dilution in immunohistochemical analysis, followed by goat anti-mouse Alexa Fluor® 488.

    See Abreview

References

This product has been referenced in:

  • Zheng C  et al. TRH Analog, Taltirelin Improves Motor Function of Hemi-PD Rats Without Inducing Dyskinesia via Sustained Dopamine Stimulating Effect. Front Cell Neurosci 12:417 (2018). Read more (PubMed: 30555300) »
  • Cuello AC  et al. Characterization and immunocytochemical application of monoclonal antibodies against enkephalins. J Histochem Cytochem 32:947-57 (1984). Read more (PubMed: 6086744) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain(striatum))
Specification
Brain(striatum)

Dr. Hyun Jin Kim

Verified customer

Submitted Apr 27 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (forebrain striatum)
Antigen retrieval step
Other
Permeabilization
Yes - 0.2% triton
Specification
forebrain striatum
Blocking step
various tested incl combinations of BSA serum, gelatin as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Heike Rebholz

Verified customer

Submitted Aug 18 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.5% triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 12 2014

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