Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Enkephalin antibody [NOC1]

Inconclusive
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (forebrain striatum)
Antigen retrieval step
Other
Permeabilization
Yes - 0.2% triton
Specification
forebrain striatum
Blocking step
various tested incl combinations of BSA serum, gelatin as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA/ PBS
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: a mouse 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
we did not get any signal using this antibody. Legend for the figure: red: pERk (rabbit) after L-DOPA stimulation, green: background for the Enkephalin antibody- no cell is visible. 20X DL striatum
Abcam response
Abcam wishes to thank the user for submitting the review. Note- ab150346 is not validated for IHC-FoFr tissue sections.

Heike Rebholz

Verified customer

Submitted Aug 18 2015

Sign up