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Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (forebrain striatum)
Antigen retrieval step
Yes - 0.2% triton
various tested incl combinations of BSA serum, gelatin as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA/ PBS
Non-Abcam antibody was used: a mouse 488
Host species: Goat
Conjugation: Alexa Fluor® 488
we did not get any signal using this antibody.
Legend for the figure: red: pERk (rabbit) after L-DOPA stimulation, green: background for the Enkephalin antibody- no cell is visible.
20X DL striatum
Abcam wishes to thank the user for submitting the review. Note- ab150346 is not validated for IHC-FoFr tissue sections.
Submitted Aug 18 2015