Leucine Aminopeptidase (LAP) Activity Assay Kit (Fluorometric) (ab234627)

Overview

  • Product name

    Leucine Aminopeptidase (LAP) Activity Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells, Tissue Homogenate
  • Assay type

    Enzyme activity
  • Sensitivity

    0.1 mU/well
  • Product overview

    Leucine Aminopeptidase (LAP) Activity Assay Kit (Fluorometric) (ab234627) provides a quick, sensitive and easy way for measuring total LAP activity in various samples such as animal tissues or cell cultures.


    In this assay, LAPs hydrolyze leucine from the fluorescent probe and the amount of fluorescent probe detected at Ex/Em 368/460 nm is used to determine the total activity of the LAP enzymes. The assay is simple to perform, high-throughput adaptable and can detect less than 0.1 mU of LAP activity.

  • Notes

    Leucine aminopeptidases (EC 3.4.11.1) (LAPs) are a diverse set of exopeptidases that catalyze the hydrolysis of leucine residues from the amino-termini of proteins or peptides. LAPs are ubiquitous enzymes present among animals, plants and prokaryotes. Previously, they were thought to typically play important roles in cell maintenance, growth and development. However, research in the recent years has identified multiple secondary functions for these enzymes in animals and microbes including transcriptional regulation and vesicle transport. Studies have implicated LAP enzymes in tumor cell proliferation, invasion and angiogenesis. Placental LAP is used as a biomarker in ovarian epithelial cancer while adipocyte-derived LAP is used as a marker of endometrial cancer cell proliferation and differentiation.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    AMC Standard (1 mM)  Yelllow 1 x 100µl
    LAP Assay Buffer WM 1 x 25ml
    LAP Positive Control  Violet 1 vial
    LAP Substrate  Blue 1 vial

Images

  • AMC standard curve.

  • Reaction kinetics of leucine aminopeptidase activity in rat liver (6.6 µg protein) and Jurkat (human T cell leukemia cell line from peripheral blood) cells (8 µg protein) using appropriate background controls.

  • Leucine aminopeptidase specific activity was calculated in rat liver and Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysates.

Protocols

References

ab234627 has not yet been referenced specifically in any publications.

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