Anti-LNPEP antibody - N-terminal (ab190255)
Key features and details
- Rabbit polyclonal to LNPEP - N-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LNPEP antibody - N-terminal
See all LNPEP primary antibodies -
Description
Rabbit polyclonal to LNPEP - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Pig, Chimpanzee, Rhesus monkey, Common marmoset -
Immunogen
Synthetic peptide within Human LNPEP aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_005566.2.
Database link: Q9UIQ6 -
Positive control
- HeLa, 293T and Jurkat whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab190255 was affinity purified using an epitope specific to LNPEP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 117 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 117 kDa. |
Target
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Function
Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain. -
Tissue specificity
Highly expressed in placenta, heart, kidney and small intestine. Detected at lower levels in neuronal cells in the brain, in skeletal muscle, spleen, liver, testes and colon. -
Sequence similarities
Belongs to the peptidase M1 family. -
Post-translational
modificationsThe pregnancy serum form is derived from the membrane-bound form by proteolytic processing.
N-glycosylated. -
Cellular localization
Cell membrane. In brain only the membrane-bound form is found. The protein resides in intracellular vesicles together with GLUT4 and can then translocate to the cell surface in response to insulin and/or oxytocin. Localization may be determined by dileucine internalization motifs, and/or by interaction with tankyrases and Secreted. During pregnancy serum levels are low in the first trimester, rise progressively during the second and third trimester and decrease rapidly after parturition. - Information by UniProt
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Database links
- Entrez Gene: 461960 Chimpanzee
- Entrez Gene: 100391174 Common marmoset
- Entrez Gene: 100073222 Horse
- Entrez Gene: 4012 Human
- Entrez Gene: 100125826 Pig
- Entrez Gene: 703949 Rhesus monkey
- Omim: 151300 Human
- SwissProt: Q9UIQ6 Human
see all -
Alternative names
- angiotensin IV receptor antibody
- AT (4) receptor antibody
- CAP antibody
see all
Images
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Detection of LNPEP by Western Blot of Immunoprecipitate.
ab190255 at 1 µg/ml labeling LNPEP in Jurkat whole cell lysate immunoprecipitated using ab190255 at 6 µg/reaction (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds. -
All lanes : Anti-LNPEP antibody - N-terminal (ab190255) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 117 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab190255 has not yet been referenced specifically in any publications.