Recombinant
RabMAb

Recombinant Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)

Overview

  • Product name

    Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712]
    See all Leukotriene A4 hydrolase/LTA4H primary antibodies
  • Description

    Rabbit monoclonal [EPR5712] to Leukotriene A4 hydrolase/LTA4H
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Leukotriene A4 hydrolase/LTA4H aa 1-100 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, T47-D, and A549 cell lysates; Human breast carcinoma tissue
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     This product was previously labelled as Leukotriene A4 hydrolase

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 69 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC.
  • Target

    Images

    • All lanes : Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : T47D cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 69 kDa

    • Immunohistochemical analysis of Leukotriene A4 hydrolase/LTA4H in paraffin-embedded Human breast carcinoma tissue using ab109434 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab109434 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for contacting us.

    Both antibodies are raised in rabbit but they are from two different clones (ab109434 from clone EPR5712 compared to ab133512 from clone EPR5713).

    There are slight variations between the two products such as the immunogen sequence, which is amino acids 35-60 for ab109434 and amino acids 575-600 for ab133512 of Human Leukotriene A4 hydrolase.

    Furthermore the products are tested in different species and applications. Antibody ab109434 reacts with rat and human but does not react with mouse and is suitable for WB, IP, IHC-P as well as Flow Cyt. Compared to that antibody ab133512 reacts with mouse, rat and human and was tested in WB, IHC-P, ICC/IF and Flow Cyt applications.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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