Recombinant
RabMAb

Recombinant Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5713] (ab133512)

Overview

  • Product name

    Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5713]
    See all Leukotriene A4 hydrolase/LTA4H primary antibodies
  • Description

    Rabbit monoclonal [EPR5713] to Leukotriene A4 hydrolase/LTA4H
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Leukotriene A4 hydrolase/LTA4H aa 550-650. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, T47D and A549 cell lysates; Human kidney tissue.
  • General notes

     

     

     This product was previously labelled as Leukotriene A4 hydrolase

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 69 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • All lanes : Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5713] (ab133512) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : T47D cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 69 kDa

    • Overlay histogram showing Jurkat cells stained with ab133512 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133512, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5713] (ab133512) at 1/5000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse heart tissue lysate
      Lane 3 : Mouse kidney tissue lysate
      Lane 4 : Mouse spleen tissue lysate
      Lane 5 : Rat brain tissue lysate
      Lane 6 : Rat heart tissue lysate
      Lane 7 : Rat kidney tissue lysate
      Lane 8 : Rat spleen tissue lysate

      Predicted band size: 69 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 minute
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Leukotriene A4 hydrolase/LTA4H with ab133512 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Tsuda S  et al. Novel mechanism of regulation of the 5-lipoxygenase/leukotriene B4 pathway by high-density lipoprotein in macrophages. Sci Rep 7:12989 (2017). Read more (PubMed: 29021582) »
    • Lin HN & O'Connor JP Immunohistochemical localization of key arachidonic acid metabolism enzymes during fracture healing in mice. PLoS One 9:e88423 (2014). IHC-P ; Mouse . Read more (PubMed: 24516658) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting us.

    Both antibodies are raised in rabbit but they are from two different clones (ab109434 from clone EPR5712 compared to ab133512 from clone EPR5713).

    There are slight variations between the two products such as the immunogen sequence, which is amino acids 35-60 for ab109434 and amino acids 575-600 for ab133512 of Human Leukotriene A4 hydrolase.

    Furthermore the products are tested in different species and applications. Antibody ab109434 reacts with rat and human but does not react with mouse and is suitable for WB, IP, IHC-P as well as Flow Cyt. Compared to that antibody ab133512 reacts with mouse, rat and human and was tested in WB, IHC-P, ICC/IF and Flow Cyt applications.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up