Product nameAnti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5713]
See all Leukotriene A4 hydrolase/LTA4H primary antibodies
DescriptionRabbit monoclonal [EPR5713] to Leukotriene A4 hydrolase/LTA4H
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Leukotriene A4 hydrolase/LTA4H aa 550-650. The exact sequence is proprietary.
- HeLa, 293T, T47D and A549 cell lysates; Human kidney tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.40 x 10 -11 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 69 kDa.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionHydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). The enzyme also has some peptidase activity.
PathwayLipid metabolism; leukotriene B4 biosynthesis.
Sequence similaritiesBelongs to the peptidase M1 family.
- Information by UniProt
- FLJ17564 antibody
- Leukotriene A(4) hydrolase antibody
- Leukotriene A-4 hydrolase antibody
All lanes : Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5713] (ab133512) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : T47D cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 69 kDa
Overlay histogram showing Jurkat cells stained with ab133512 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133512, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
All lanes : Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5713] (ab133512) at 1/5000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Predicted band size: 69 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Leukotriene A4 hydrolase/LTA4H with ab133512 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Tsuda S et al. Novel mechanism of regulation of the 5-lipoxygenase/leukotriene B4 pathway by high-density lipoprotein in macrophages. Sci Rep 7:12989 (2017). Read more (PubMed: 29021582) »
- Lin HN & O'Connor JP Immunohistochemical localization of key arachidonic acid metabolism enzymes during fracture healing in mice. PLoS One 9:e88423 (2014). IHC-P ; Mouse . Read more (PubMed: 24516658) »