Anti-Leukotriene B4 Receptor/BLT antibody (ab133185)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Leukotriene B4 Receptor/BLT antibody
See all Leukotriene B4 Receptor/BLT primary antibodies -
Description
Rabbit polyclonal to Leukotriene B4 Receptor/BLT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow
Does not react with:
Mouse -
Immunogen
Synthetic peptide corresponding to Human Leukotriene B4 Receptor/BLT aa 331-352.
Sequence:ALEPGPSESLTASSPLKLNELN
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Positive control
- Human spleen and tonsil tissues.
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General notes
This product was previously labelled as Leukotriene B4 Receptor
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% Tris buffered saline, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab133185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/200. Predicted molecular weight: 38 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC | Use at an assay dependent concentration. |
Target
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Function
Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response. -
Tissue specificity
Expressed at highest levels in heart, skeletal muscle and at lower levels in brain and liver. High level of expression in lymphoid tissues. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsPhosphorylated by GRK6 upon leukotriene B4 binding; which promotes desensitization. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 613482 Cow
- Entrez Gene: 1241 Human
- Omim: 601531 Human
- SwissProt: Q15722 Human
- Unigene: 567248 Human
- Unigene: 727320 Human
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Alternative names
- BLT antibody
- BLT1 antibody
- BLTR antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody (ab133185)Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Leukotriene B4 Receptor/BLT with ab133185 antibody at a concentration of 5 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody (ab133185)Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling Leukotriene B4 Receptor/BLT with ab133185 antibody at a concentration of 5 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Protocols
Datasheets and documents
References
ab133185 has not yet been referenced specifically in any publications.
