Recombinant Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7113] to Leukotriene B4 Receptor/BLT
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113]
See all Leukotriene B4 Receptor/BLT primary antibodies -
Description
Rabbit monoclonal [EPR7113] to Leukotriene B4 Receptor/BLT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human thymus, Human fetal lung and A549 cell lysates; Human breast carcinoma and tonsil tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.96 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7113 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response. -
Tissue specificity
Expressed at highest levels in heart, skeletal muscle and at lower levels in brain and liver. High level of expression in lymphoid tissues. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsPhosphorylated by GRK6 upon leukotriene B4 binding; which promotes desensitization. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1241 Human
- Omim: 601531 Human
- SwissProt: Q15722 Human
- Unigene: 567248 Human
- Unigene: 727320 Human
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Alternative names
- BLT antibody
- BLT1 antibody
- BLTR antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Leukotriene B4 Receptor/BLT with ab131041 antibody at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041) at 1/10000 dilution
Lane 1 : Human thymus lysate
Lane 2 : Human fetal lung tissue lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa -
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Leukotriene B4 Receptor/BLT with ab131041 antibody at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab131041 has been referenced in 2 publications.
- Su KK et al. Five-lipoxygenase-activating protein-mediated CYLD attenuation is a candidate driver in hepatic malignant lesion. Front Oncol 12:912881 (2022). PubMed: 35978827
- Jeon WK et al. The proinflammatory LTB4/BLT1 signal axis confers resistance to TGF-ß1-induced growth inhibition by targeting Smad3 linker region. Oncotarget 6:41650-66 (2015). IHC . PubMed: 26497676