Product nameAnti-Leukotriene B4 Receptor/BLT antibody [EPR7113]
See all Leukotriene B4 Receptor/BLT primary antibodies
DescriptionRabbit monoclonal [EPR7113] to Leukotriene B4 Receptor/BLT
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
corresponding to Human Leukotriene B4 Receptor/BLT aa 300-400 (C terminal).
Database link: Q15722
- Human thymus, Human fetal lung and A549 cell lysates; Human breast carcinoma and tonsil tissues
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Leukotriene B4 Receptor
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.96 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
Our Abpromise guarantee covers the use of ab131041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionReceptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response.
Tissue specificityExpressed at highest levels in heart, skeletal muscle and at lower levels in brain and liver. High level of expression in lymphoid tissues.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsPhosphorylated by GRK6 upon leukotriene B4 binding; which promotes desensitization.
Cellular localizationCell membrane.
- Information by UniProt
- BLT antibody
- BLT1 antibody
- BLTR antibody
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Leukotriene B4 Receptor/BLT with ab131041 antibody at 1/50 dilution.
All lanes : Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041) at 1/10000 dilution
Lane 1 : Human thymus lysate
Lane 2 : Human fetal lung lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Leukotriene B4 Receptor/BLT with ab131041 antibody at 1/50 dilution.
Equilibrium disassociation constant (KD)
Learn more about KD
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