Recombinant
RabMAb

Recombinant Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041)

Overview

  • Product name

    Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113]
    See all Leukotriene B4 Receptor/BLT primary antibodies
  • Description

    Rabbit monoclonal [EPR7113] to Leukotriene B4 Receptor/BLT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human Leukotriene B4 Receptor/BLT aa 300-400 (C terminal).
    Database link: Q15722

  • Positive control

    • Human thymus, Human fetal lung and A549 cell lysates; Human breast carcinoma and tonsil tissues
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Leukotriene B4 Receptor

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response.
    • Tissue specificity

      Expressed at highest levels in heart, skeletal muscle and at lower levels in brain and liver. High level of expression in lymphoid tissues.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Post-translational
      modifications

      Phosphorylated by GRK6 upon leukotriene B4 binding; which promotes desensitization.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • BLT antibody
      • BLT1 antibody
      • BLTR antibody
      • Chemoattractant receptor-like 1 antibody
      • Chemokine receptor like 1 antibody
      • CMKRL1 antibody
      • G protein coupled receptor 16 antibody
      • G-protein coupled receptor 16 antibody
      • GPR16 antibody
      • Leukotriene B4 G Protein coupled receptor antibody
      • Leukotriene B4 receptor 1 antibody
      • Leukotriene B4 receptor antibody
      • LT4R1_HUMAN antibody
      • LTB4 R 1 antibody
      • LTB4-R 1 antibody
      • LTB4-R1 antibody
      • LTB4R 1 antibody
      • Ltb4r antibody
      • LTB4R1 antibody
      • LTBR1 antibody
      • P2RY7 antibody
      • P2Y purinoceptor 7 antibody
      • P2Y7 antibody
      • Purinergic receptor P2Y G protein coupled 7 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Leukotriene B4 Receptor/BLT with ab131041 antibody at 1/50 dilution.

    • All lanes : Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041) at 1/10000 dilution

      Lane 1 : Human thymus lysate
      Lane 2 : Human fetal lung lysate
      Lane 3 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 38 kDa
      Observed band size: 38 kDa

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Leukotriene B4 Receptor/BLT with ab131041 antibody at 1/50 dilution.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Jeon WK  et al. The proinflammatory LTB4/BLT1 signal axis confers resistance to TGF-ß1-induced growth inhibition by targeting Smad3 linker region. Oncotarget 6:41650-66 (2015). IHC . Read more (PubMed: 26497676) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131041.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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