Overview

  • Product name

  • Description

    Rabbit polyclonal to Leupaxin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Leupaxin aa 115-129. The immunogen sequence differs from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.
    Sequence:

    KKHLPDKQDHKASLD


    Database link: O60711

  • Positive control

    • Jurkat cell and rat thymus tissue lysates; mouse and rat spleen tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab181621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 43 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Leupaxin is preferentially expressed in hematopoietic cells and is most homologous to the focal adhesion protein, paxillin. It may function in cell type specific signaling by associating with PYK2, a member of focal adhesion kinase family. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by tyrosine kinase activity.
  • Cellular localization

    Cell Membrane, Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • LDLP antibody
    • LDPL antibody
    • leupaxin antibody

Images

  • All lanes : Anti-Leupaxin antibody (ab181621) at 0.5 µg/ml

    Lane 1 : rat thymus tissue lysate
    Lane 2 : Jurkat cell lysate

    Predicted band size: 43 kDa

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue, labeling Leupaxin using ab181621 at 1 μg/ml.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue, labeling Leupaxin using ab181621 at 1 μg/ml.

References

This product has been referenced in:

  • Shi J  et al. Regulation of ß-catenin transcription activity by leupaxin in hepatocellular carcinoma. Tumour Biol N/A:N/A (2015). Read more (PubMed: 26361959) »
See 1 Publication for this product

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