Key features and details
- Rabbit polyclonal to Leupaxin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Leupaxin antibody
See all Leupaxin primary antibodies
DescriptionRabbit polyclonal to Leupaxin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- Jurkat cell and rat thymus tissue lysates; mouse and rat spleen tissues.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab181621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 43 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceLeupaxin is preferentially expressed in hematopoietic cells and is most homologous to the focal adhesion protein, paxillin. It may function in cell type specific signaling by associating with PYK2, a member of focal adhesion kinase family. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by tyrosine kinase activity.
Cellular localizationCell Membrane, Cytoplasmic and Nuclear
- LDLP antibody
- LDPL antibody
- leupaxin antibody
All lanes : Anti-Leupaxin antibody (ab181621) at 0.5 µg/ml
Lane 1 : rat thymus tissue lysate
Lane 2 : Jurkat cell lysate
Predicted band size: 43 kDa
Immunohistochemical analysis of paraffin-embedded rat spleen tissue, labeling Leupaxin using ab181621 at 1 μg/ml.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue, labeling Leupaxin using ab181621 at 1 μg/ml.
ab181621 has been referenced in 1 publication.
- Shi J et al. Regulation of ß-catenin transcription activity by leupaxin in hepatocellular carcinoma. Tumour Biol N/A:N/A (2015). PubMed: 26361959